Chinese Journal of Tissue Engineering Research ›› 2014, Vol. 18 ›› Issue (49): 8001-8006.doi: 10.3969/j.issn.2095-4344.2014.49.023

Previous Articles     Next Articles

Inhibitory effect of colchicine on transforming growth factor β1/Smads pathway in rat models of chronic pancreatitis   

Lu Hong-wei, Zhang Ya-fei, Ji Hong, Wang Jin-long, Li Yi-ming   

  1. Department of General Surgery, Second Affiliated Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China
  • Revised:2014-09-19 Online:2014-11-30 Published:2014-11-30
  • Contact: Li Yi-ming, Professor, Doctoral supervisor, Department of General Surgery, Second Affiliated Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China
  • About author:Lu Hong-wei, M.D., Associate chief physician, Department of General Surgery, Second Affiliated Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China
  • Supported by:

    the Research Project of Social Development in Shaanxi Province of China, No. 2007k14-02(17)

Abstract:

BACKGROUND: Pancreatic stellate cells transforming growth factor β1/Smads signaling pathway activation is probably a main molecular mechanism of pancreatic fibrosis. If this pathway can be blocked, the progression of fibrosis of tissues with chronic pancreatitis will be inhibited.

OBJECTIVE: To study the inhibitory effect of colchicine on transforming growth factor β1/Smads pathway in chronic pancreatitis rat models.
METHODS: Healthy male Sprague-Dawley rats were randomly divided into colchicines-treated group and chronic pancreatitis group. After successful establishment of rat models of chronic pancreatitis, the rats in the colchicines-treated group were intraperitoneally injected with colchicine 150 μg/kg daily. The rats in the chronic pancreatitis group were intraperitoneally injected with equal volume of physiological saline daily. Pancreatic tissues were collected after 3 months. Hematoxylin-eosin staining was used to observe histopathological changes of pancreatic tissue. Immunohistochemical staining was used to detect the expressions of transforming growth factor β1 in pancreatic tissue. Western blot assay was utilized to detect the expressions of P-Smad2, P-Smad3 and α-SMA protein in pancreatic stellate cells.
RESULTS AND CONCLUSION: Hematoxylin-eosin staining results revealed that compared with the colchicines-treated group, glandular tissue had reduced, while fibrous connective tissue and inflammatory cells had increased obviously and replaced the pancreatic gland tissue in the chronic pancreatitis group. Immunohistochemical staining results demonstrated that the expression levels of transforming growth factor β1 and the index of positive cells were significantly lower in the colchicines-treated group than those in the chronic pancreatitis group (P < 0.05). Western blot assay results revealed that the results of P-Smad2/β-actin, P-Smad3/β-actin and α-SMA/β-actin in pancreatic stellate cells were significantly lower in the chronic pancreatitis group than those in the colchicines-treated group (P < 0.05). Results suggested that colchicine could inhibit the activity of transforming growth factor β1/Smads pathway and pancreatic tissue fibrosis in chronic pancreatitis rats. Therefore, colchicine can be used as a new candidate therapeutic scheme for chronic pancreatitis fibrosis.


中国组织工程研究
杂志出版内容重点:肾移植肝移植移植;心脏移植;组织移植;皮肤移植;皮瓣移植;血管移植;器官移植组织工程


全文链接:

Key words: transforming growth factor beta 1, smad2 protein, smad3 protein, pancreatitis, chronic

CLC Number: