Compliance of ischemic tissue to stem cells-derived vessels for secondary revascularization

doi:10.3969/j.issn.2095-4344.2014.45.021

Abstract

Abstract:

BACKGROUND: To explore the methods for secondary limb revascularization after endovascular repair is one of the most urgent research topics.

OBJECTIVE: To investigate the biological mechanism about the transplantation of autologous peripheral blood stem cells to lead and perfect the effect of ischemic tissue for secondary revascularization.

METHODS: Forty-two patients with critical low limb ischemia were selected and treated with endovascular repair as first revascularization and transplantation of autologous peripheral blood stem cells as secondary revascularization. The secondary revascularization was carried out at 3 months after the first blood flow remodeling. The patients receiving secondary revascularization were followed for 4 years.

RESULTS AND CONCLUSION: After transplantation of autologous peripheral blood stem cells, the patients complained that the rest pain of the lower leg was relieved obviously and intermittent claudication distance was significantly lengthened. Limb salvage rate was 100%. Skin temperature index was 1.6±0.3, transcutaneous oxygen pressure was (5.00±1.26) kPa, ankle-brachium index was 0.9±0.2, photoplethysmograpy index was 0.8±0.1, saturation of blood oxygen was (79.4±20.4)%, and digital subtraction angiography score was (1.3±0.2)points. After transplantation, local blood flow indicators of the low limbs were positively correlated to local symptom indicators of the limbs (0.6< r <0.7, P < 0.05). These results strongly suggest that the stem cells-derived neovascularization can offer effective and permanent blood flow perfusions to the ischemic tissue, and this biological effect play an important role in secondary revascularization of the ischemic tissue.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: lower extremity, ischemia, angioplasty, peripheral blood stem cell transplantation, neovascularization, physiologic

CLC Number:

R394.2

He Xiao-ming, Yang Yong, Wan Jia. Compliance of ischemic tissue to stem cells-derived vessels for secondary revascularization[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(45): 7333-7336.

Figures/Tables 1

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