Variation of tumor necrosis factor-alpha during acute rejection after liver transplantation in a rhesus monkey

doi:10.3969/j.issn.2095-4344.2014.36.003

Abstract

Abstract:

BACKGROUND: Tumor necrosis factor-α is an inflammatory cytokine involved in the immune response and increasing graft antigen expression.
OBJECTIVE: To investigate the relationship between tumor necrosis factor-α in the liver tissue and acute rejection after liver transplantation in a rhesus monkey.
METHODS: Liver transplant models in rhesus monkey were constructed by the improved vascular dual cuff, supporting tube of biliary tract and artery anastomosis method. The successful models were randomly divided into experimental group (no immunosuppressant treatment in perioperative period) and control group (treated by immunosuppressant in perioperative period). Then the blood samples and liver tissue were collected at 6, 12, 24, and 72 hours after surgery. Allograft rejections of liver transplantation were monitored by liver function tests, hematoxylin-eosin staining and Banff score. Finally, the expression level of tumor necrosis factor-α was detected by western blot analysis and immunohistochemistry technique.
RESULTS AND CONCLUSION: The expression of tumor necrosis factor-α in the experimental group and control group began to increase at 6 hours, reached the peak at 12 hours, and then decreased at 24-72 hours. The changes of expression level were the most obvious in the experimental group. At 6, 12, 24 and 72 hours, the expression of tumor necrosis factor-α in the experimental group was significantly higher than that in the control group (P < 0.05). This change appeared earlier than pathological changes in the liver and liver function. Variations in the expression of tumor necrosis factor-α after liver transplantation have important implications for early diagnosis of acute rejection after liver transplantation.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: liver transplantation, acute rejection, tumor necrosis factor-α

CLC Number:

R318

Zhang Xi-bing, Ran Jiang-hua, Liu Jing, Zhang Sheng-ning, Li Lai-bang, Chen Yi-ming, Gao Yang, Li Wang, Li Li. Variation of tumor necrosis factor-alpha during acute rejection after liver transplantation in a rhesus monkey[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(36): 5752-5757.

Figures/Tables 4

References

[1] Goronzy JJ, Weyand CM. T-cell co-stimulatory pathways in autoimmunity. Arthritis Research and Therapy. 2008;10(1, article S3).
[2] Alex Bishop G, Bertolino PD, Bowen DG,et al.Tolerance in liver transplantation.Best Pract Res Clin Gastroenterol.2012;26(1):73-84.
[3] Rook M, Rand E.Predictors of long-term outcome after liver transplant.Curr Opin Organ Transplant.2011;16(5):499-504.
[4] Li DH, Havell EA, Brown CL, et al.Woodchuck lymphotoxin-alpha, beta and tumor necrosis factor genes: structure, characterization and biological activity.Gene.2000 25;242(1-2):295-305.
[5] Nedwin GE, Naylor SL, Sakaguelli AY, et al. Human lymphotoxin and tumorNecerosis factor genes: structure、homology and chromosomal localization. Nueleie Acids Res.1985;13(17):6361.
[6] Bagul A.Ischaemic/reperfusion injury: Role of infliximab.World J Transplant.2012;2(3)35-40.
[7] El-Gezawy EM, Eldin EN, Mohamed WS,et al.Tumor necrosis factor-alfa and monocyte chemoattractant protein-1 gene polymorphisms in kidney transplant recipients.Saudi J Kidney Dis Transpl.2013;24(4):688-695.
[8] Roek CS,Lowry SF. Tumor necrosis factor-alpha.J Surg Res. 1991;51(5):434-445.
[9] The Ministry of Science and Technology of the People’s Republic of China. Guidance Suggestions for the Care and Use of Laboratory Animals. 2006-09-30.
[10] Allain JE, Mahieu-Caputo D, Loux N, et al.Impronement of Orthotopic Liver Transplantation Model in Rhesus Monkey.Chin J Bases Clin General Surg.2010;3(4):212-219.
[11] 冉江华,李铸,刘静,等.二袖套法建立恒河猴原位肝移植的稳定模型[J].中国组织工程研究,2011,15(5):62-768.
[12] Busuttil RW, Lake JR.Role of tacrolimus in the evolution of liver transplantation. Transplantation.2004;77(9 suppl):S44-51.
[13] Racusen LC,Halloran PF,Solez K.Banff 2003 meeting report:new diagnostic insights and standards.Am J Transplant.2004;4(10):1562-1566.
[14] Solez K. History of the Banff classification of allograft pathology as it approaches its 20th year.Curr Opin Organ Transplant.2010;15(1):49-51.
[15] Rüdiger T, Höfler H, Kreipe HH,et al.Quality assurance in immunohistochemistry: results of an interlaboratory trial involving172 pathologists.Am J Surg Pathol.2002;26(7): 873-882.
[16] Dickson C.Protein techniques: immunoprecipitation, in vitro kinase assays, and Western blotting.Methods Mol Biol.2008;461:735-744.
[17] Matsunaga K, Mashiba H. Augmentation of TNF-and lymphotoxin-mediated cytotoxic effect in the combined use of ACNU and involvement of oxygen free radicals.Gan To Kagaku Ryoho.1990;17(3pt2):452-458.
[18] Matsubara N,Fuehimoto S,Orita K.Differing roles of Protein kinase C on the antiProliferative effects of tumor necrosis factor alpha and beta on LoVo cell.Pathobiology.1990;58(3):168-171.
[19] Koga Y, Matsuzaki A, Suminoe A,al et.Neutrophil-derived TNF-related apoptosis-inducing ligand (TRAIL): a novel mechanism ofantitumor effect by neutrophils.Cancer Res. 2004; 64(3):1037-1043.
[20] Futosi K, Fodor S, Mócsai A. Neutrophil cell surface receptors and their intracellular signal transduction pathways. Int Immunopharmacol.2013;17(3):638-650.
[21] Bradley JR. TNF-mediated inflammatory disease. J Pathol. 2008;214(2):149-160.
[22] Girnita DM, Webber SA, Zeevi A. Clinical impact of cytokine and growth factor genetic polymorphisms in thoracic organ transplantation.Clin Lab Med.2008;28(3):423-440.
[23] Cardoni RL, Prigoshin N, Tambutti ML. Regulatory cytokines in the response to the allogeneic renal transplant. Medicina (B Aires).2005;65(1):54-62.
[24] Kita Y, Iwaki Y, Noguehi K, et al. Daily serum infianunatory cytokine (turnor necrosis factor-a, interleukin-6) monitoring in liver transplantation focusing on all graft rejection:a five-case report. Transplant Proc.1996;28(3):1237.
[25] Heidt S, San Segundo D, Shankar S.Peripheral blood sampling for the detection of allograft rejection: biomarker identification and validation.Transplantation.2011;92(1):1-9.
[26] Imagawa DK, Millis JM, Olthoff KM, et al.The role of tumor necrosis factor in allograft rejection. TransPlantation.1990; 50(2): 219-225.
[27] Pascher A, Klupp J.Biologics in the treatment of transplant rejection and ischemia/reperfusion injury: new applications for TNFalpha inhibitors? BioDrugs.2005;19(4):211-231.
[28] Rokahr KL, MeCaughan GW, NaPoli J, et al. Quantitation of eytokine Mrna Expression in biopsies from human liver allograft by a novel reverse transeriptase/polymerase chain reaetion method. Trans Plant Proe.1995;27(3):2148.
[29] Hoffmann MW, Wonigeit K, Steinlloff G, et al.Production of cytokines(TNF-α,IL-lβ)And endothelial cell aetivation in human liver allograft rejeetion.TransPlantation.1993;55(2):329-335.
[30] Basista MH, Stieffenllofer A, Kim DG, et al. Proeoagulant aetivity and tumor necrosis factor in rat hepatic allograft rejection.HePatology.1991;14(5):883-887.
[31] Adams DH, Garner C, Neuberger JM. Serum tumor necrosis factor alpha in Patients following liver transplantation. Trans Plant Proe.1990;22(5):2310.
[32] Imagawa DK, Millis JM, Olthoff KM,et al.The role of tumor necrosis factor in allograft rejection. I. Evidence that elevated levels of tumor necrosis factor-alpha predict rejection following orthotopic liver transplantation.Transplantation.1990;50(2):219-225.
[33] Fugger R, Hamilton G, Steininger R, et al. Intra operative estimation of endotoxin,TNF-a,and IL-6 in orthotopic liver transplantation and the irrelation to rejection and post operative infection. TransPlantation.1991;52(2):302.
[34] Bathgate AJ, Praviea V, Perrey C, et al. The effect of polymorphisms in tumor necrosis factor-alpha, interleukin-10, and transforming growth factor beta genes in acut hepatic allograft rejeetion. Transplantation.2000;69(7):1514.
[35] Flach R, Speidel N, Flohé S, et al. Analysis of intragraft cytokine expression during early reperfusion after liver transplantation using semi-quantitative RT-PCR.Cytokine.1998;10(6):445-451.