Fingolimod hydrochloride suppresses inflammatory reaction of blood vessels after balloon injury of the carotid artery

doi:10.3969/j.issn.2095-4344.2014.11.012

Abstract

Abstract:

BACKGROUND: Inflammatory factor plays an important role in restenosis after balloon injury. Sphingosine1-phosphate receptor 1 can enhance the expression of inflammatory factor and promote development and progression of this pathological process.
OBJECTIVE: To observe the expression of the inflammatory factors and sphingosine1-phosphate receptor 1 after balloon injury of the rat carotid artery and effects of fingolimod hydrochloride on reducing inflammatory reaction.
METHODS: Sixty Sprague-Dawley rats were equally and randomly divided into four groups. In the blank control group and negative control group, left common carotid artery was only isolated, and left external carotid artery was ligated. In the balloon injury group and drug intervention group, rat models of carotid artery injury were established by balloon injury on the left common carotid artery. In the negative control and drug intervention groups, the rats were intraperitoneally injected with fingolimod hydrochloride 1 mg/kg. In the blank control and balloon injury groups, the rats were intraperitoneally injected with an equal volume of saline. Samples were collected at 3, 7 and 21 days.
RESULTS AND CONCLUSION: Hematoxylin-eosin staining showed that the proliferation of blood vessel was remarkable in the balloon injury group, but attenuated in the drug intervention group. The appearance of blood vessels was normal in the blank control group and negative control group. Real-time fluorescent quantitative PCR revealed that cyclooxygenase 2 and prostaglandin E2 mRNA expression levels were significantly lower in the drug intervention group than those in the balloon injury group at 7 days (P < 0.05). Cyclooxygenase 2 and prostaglandin E2 mRNA expression levels were significantly higher in the balloon injury group and drug intervention group than those in the blank control group and negative control group at the same time point (P < 0.05). Western blot assay results revealed that sphingosine1-phosphate receptor 1 expression was high in early stage of injury, and then reduced in late stage of injury. In particular, protein expression further decreased after drug intervention. Results indicated that fingolimod hydrochloride suppressed inflammatory reaction of injured blood vessels and lessened the stenosis of injured blood vessels by regulating cyclooxygenase 2 and prostaglandin E2 mRNA expression using sphingosine1-phosphate receptor 1.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: common carotid artery, sphingosine, cyclooxygenase 2, dinoprostone

CLC Number:

R318

Liu Liang, Bai Feng, Sun Shou-gang, Xu Guang-li, Hu Hao, Guo Xue-ya. Fingolimod hydrochloride suppresses inflammatory reaction of blood vessels after balloon injury of the carotid artery[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(11): 1712-1717.

Figures/Tables 3

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