Response of gastric-related neurons in the hypothalamic paraventricular nucleus to acupuncture at Neiguan and Zusanli in a rat model of gastric distension

doi:10.3969/j.issn.2095-4344.2014.05.004

Abstract

Abstract:

BACKGROUND: Previous studies concerning neuromechanism of acupuncture for regulating gastric function mainly focused on peripheral nerve, involving in central nervous system. In particular, there are few studies addressing higher central nervous system.

OBJECTIVE: To explore the effects of acupuncture at Neiguan (PC6) and Zusanli (ST36) on gastric-related neurons in the hypothalamic paraventricular nucleus.

METHODS: Using microelectrode extracellular recording technique, gastric related neurons in the paraventricular nucleus were found in gastric distension rats. Stimulation was conducted for 30 seconds by hand acupuncture at Neiguan and Zusanli. The effects of acupuncture on gastric-related neurons in the paraventricular nucleus were observed.

RESULTS AND CONCLUSION: The discharge of 109 neurons in the hypothalamic paraventricular nucleus of 60 rats was recorded. A total of 56 gastric-related neurons of the 109 neurons were observed. The number of neurons response to acupunctures at Zusanli, Neiguan, Pishu (BL20) and Weishu (BL21) were respectively 44, 47, 29, and 33, with the reaction proportion of 78.57%, 83.47%, 51.79% and 58.93%. Results suggested that there exist gastric distension stimulation and acupuncture stimulations from neurons soma to visceral reaction in the hypothalamic paraventricular nucleus, which simultaneously participates in the regulation of acupuncture on the gastric function.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: paraventricular hypothalamic nucleus, neurons, gastric dilatation, vagus nerve, acupuncture points

CLC Number:

R318

Chen Shu, Yong Chun-yan, Chen Heng, Chu Xiao, Zhang Chao, Tan Cheng, Ye Lan, Li Jiang-shan. Response of gastric-related neurons in the hypothalamic paraventricular nucleus to acupuncture at Neiguan and Zusanli in a rat model of gastric distension[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(5): 675-680.

Figures/Tables 3

References

[1] 高颖,段晓英,周丹.针灸调节下丘脑-垂体-肾上腺轴的研究进展[J].中医药信息, 2006,23(3):21-22.

[2] 张咏梅,张建福,魏尔清.下丘脑室旁核对胃的调控[J].国际麻醉学与复苏杂志,2006,27(6):373-376.

[3] 李江山,常小荣,严洁,等.电针大鼠"四白"穴和胃扩张传入信息在孤束核的汇聚[J].针刺研究,2005,30(1):39-42.

[4] 李江山,严洁,何军峰,等.针刺内关、足三里等穴对大鼠孤束核神经元放电的影响[J].湖南中医药大学学报,2007,27(3):55-58.

[5] 何军峰,刘健华,严洁,等.大鼠头面部腧穴和胃扩张传入信息在孤束核的汇聚[J].中国中医药信息杂志,2005,12(2):30-32.

[6] Luiten PG, ter Horst GJ, Karst H,et al.The course of paraventricular hypothalamic efferents to autonomic structures in medulla and spinal cord.Brain Res. 1985;329 (1-2):374-378.

[7] 李江山,严洁,何军峰,等.针刺对胃扩张模型大鼠孤束核神经元放电的影响[J].中国医药导报,2009,6(8):19-21.

[8] Paxions G,Watsons C.The rat brain in stereotaxic coordinate[M].London :Academic Press,1982:136-145.

[9] 张昌云,谭奇纹.经脉-脏腑相关性的认识[J].甘肃中医,2007, 20(9): 5-7.

[10] 周逸平.经脉-脏腑相关是经络理论的核心[J].针刺研究,1999, 24(3): 238-241.

[11] 印大中,赵林立.从"植物神经生物学"和动物神经进化看经络的生物学本质[J].世界科学技术-中医药现代化,2009,11(5): 670-678.

[12] 李筱媛,李军,许明辉,等.脑功能成像在针刺研究中的应用进展[J].上海针灸杂志,2008,27(8):48-50.

[13] 孙国杰,王述菊,杜艳军,等.不同穴位对胃运动异常大鼠双相调节效应研究[J].中国针灸,2007,27(11):839-842.

[14] 赵晏.针灸经络的外周神经生物学机理探讨[J].针刺研究,2006, 31(6):329.

[15] 刘伟,朱贤立,丰育功,等.下丘脑室旁核在调节水、钠代谢神经网络中的作用[J].国外医学:神经病学神经外科学分册,2003, 30(3): 208-211.

[16] Rogers RC, Hermann GE.Oxytocin, oxytocin antagonist, TRH, and hypothalamic paraventricular nucleus stimulation effects on gastric motility.Peptides. 1987;8(3):505-513.

[17] Morrow NS, Novin D, Garrick T. Microinjection of thyrotropin-releasing hormone in the paraventricular nucleus of the hypothalamus stimulates gastric contractility.Brain Res. 1994;644(2):243-250.

[18] 张文文.室旁核注射催产素对大鼠胃缺血-再灌注损伤的保护作用及其机制的研究[D].徐州:徐州医学院,2007.

[19] 李慧.应激对大鼠延髓和下丘脑胃机能调控中枢神经路影响的研究[D].济南:山东师范大学,2010.

[20] 张咏梅,张建福,陈幽婷.胃缺血/再灌注损伤引起大鼠脑室旁核及孤束核c-fos表达[J].中国应用生理学杂志,2004,20(1):20-23.

[21] Zhang JF, Zheng F.The role of paraventricular nucleus of hypothalamus in stress-ulcer formation in rats.Brain Res. 1997; 761(2):203-209.

[22] Kannan H, Yamashita H.Connections of neurons in the region of the nucleus tractus solitarius with the hypothalamic paraventricular nucleus: their possible involvement in neural control of the cardiovascular system in rats.Brain Res. 1985; 329(1-2):205-212.

[23] 张咏梅,张建福,陈幽婷,等.胃缺血/再灌注损伤引起大鼠脑室旁核及孤束核c-fos表达[J].中国应用生理学杂志, 2004,20(1):20- 23.

[24] 陈泽斌,王述菊,王亚文,等.损毁室旁核及孤束核对电针内关穴抗家兔心肌缺血作用的影响[J].中国中医急症,2005,14(8):759- 761.

[25] Pagani FD, Norman WP, Kasbekar DK,et al. Localization of sites within dorsal motor nucleus of vagus that affect gastric motility.Am J Physiol. 1985;249(1 Pt 1):G73-84.

[26] 程世斌,罗天锡,卢光启,等.大鼠胃迷走感觉纤维的中枢投射-光镜电镜研究[J].中山医科大学学报,1993,14(7):252-255.

[27] 王光明,张建福,乔伟丽,等.下丘脑室旁核微量注射ghrelin经迷走神经增强清醒大鼠胃运动及胃电活动[J].徐州医学院学报,2009, 29(12):791-795.

[28] Dong YX, Han ZA, Xiong KH,et al.Fos expression in serotonergic midbrain neurons projecting to the paraventricular nucleus of hypothalamus after noxious stimulation of the stomach: a triple labeling study in the rat. Neurosci Res. 1997;27(2):155-160.

[29] Holstege G.Some anatomical observations on the projections from the hypothalamus to brainstem and spinal cord: an HRP and autoradiographic tracing study in the cat.J Comp Neurol. 1987;260(1):98-126.

[30] van der Kooy D, Koda LY, McGinty JF,et al.The organization of projections from the cortex, amygdala, and hypothalamus to the nucleus of the solitary tract in rat.J Comp Neurol. 1984; 224(1):1-24.

[31] Willett CJ, Rutherford JG, Gwyn DG,et al.Projections between the hypothalamus and the dorsal vagal complex in the cat: an HRP and autoradiographic study.Brain Res Bull. 1987;18(1): 63-71.

[32] 曹琦,钱雪景,赵乐章,等.损毁迷走神经背核翻转室旁核降低胃内压的观察[J].安徽医科大学学报,1994,29(1):22-24.