Effects of cyclic stretch on in vitro culture-tensile stimulation models of myoblasts

doi:10.3969/j.issn.2095-4344.2014.05.003

Abstract

Abstract:

BACKGROUND: Endoplasmic reticulum stress participates in the occurrence and development of many diseases, such as atherosclerosis, diabetes, and Alzheimer’s disease. GRP78 is a marker of endoplasmic reticulum stress. The expression of GRP78 reflects the degree of endoplasmic reticulum stress.

OBJECTIVE: To investigate the effect of cyclic stretch on GRP78 expression of L6 rat myoblasts, and to identify the relationship between cyclic stretch and endoplasmic reticulum stress.

METHODS: In vitro culture-tensile stimulation models of myoblasts of L6 rats were established successfully. The expression of GRP78 of myoblasts exposed to cyclic stretch was determined by reverse transcription-PCR and western blot assay. Stretch groups were subjected to 15% surface elongation at a frequency of 10 cycles per minute, over a period of 1, 6, 12 and 24 hours. Cells were simultaneously seeded on a plate in the control and experimental groups with no stimulation.

RESULTS AND CONCLUSION: The expression of GRP78 mRNA was continuously elevated over time after stretched treatment, and significant differences were detected as compared with the control group (P < 0.05). GRP78 protein expression began to increase at 1 hour after stretched treatment, was significantly increased at 6 hours, peaked at 24 hours, and significant differences were visible as compared with the control group (P < 0.05). In conclusion, cyclic stretch induced the occurrence of endoplasmic reticulum stress, which was enhanced with prolonged time. However, prolonged stretch caused severe endoplasmic reticulum stress and leaded to apoptosis of myoblasts.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: endoplasmic reticulum, myoblasts, apoptosis, immunoglobulin heavy chains, mechanics

CLC Number:

R318

Zhang Qiang, Wang Hong-ling, Ding Xian, Xia Chen-lei, Liu Li-juan, Wang Shuang-yu, Li Jian-ping, He Miao, Sun Wen-na, Yan Xiao, Liu Wen, Zhang Yue, Yao Ru-yong, Yuan Xiao. Effects of cyclic stretch on in vitro culture-tensile stimulation models of myoblasts[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(5): 669-674.

Figures/Tables 4

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