Radiation metabolomics of minimally invasive urine biomarkers for X-ray radiation exposure in mice

doi:10.3969/j.issn.2095-4344.2013.46.012

Abstract

Abstract:

BACKGROUND: Single hematology analysis can only reflect the body injury at a certain time point after radiation damage, but cannot reflect the longer-term cumulative status after radiation damage.
OBJECTIVE: To identify the biomarkers in blood and urine in mice after radiation damage with metabolomics method based on nuclear magnetic resonance hydrogen spectrum.
METHODS: Forty-eight mice were randomly divided into four groups and received 0 (sham radiation), 3, 9 and 27 Gy radiation. The blood samples were collected at 24 hours and 5 days after radiation. Another 36 mice were collected and divided into three groups and received 0 (sham radiation), 9 and 27 Gy radiation, then the urine samples were collected at 2 days before radiation and 5 days after radiation for 24 hours. The blood and urine samples were analyzed with proton nuclear magnetic resonance spectroscopy.
RESULTS AND CONCLUSION: The content of aspartic aminotransferase and alanine aminotransferase in blood plasma maintained a stable level after 3- or 9-Gy X-ray radiation, but the level of alkaline phosphatase in blood plasma was increased significantly after 9-Gy radiation, which indicating that low-dose head radiation may cause increased radiation damage and repair. The level of total superoxide dismutase in blood plasma was significantly decreased at 5 days after radiation which indicating that head radiation in mice could cause systemic oxidative stress. Meanwhile, the N-hexamolglycine and -thymidine level in the urine samples was significantly increased after different doses X-ray radiation, which can be used as the radiation damage markers in urine samples after head radiation. The 3-hydroxy-2-methylbenzoic acid 3-O-sulfate level in urine samples was increased by 2.5 times after received 9-Gy radiation, which can be used as the specific markers of middle- and low-dose radiation damage; the level of taurine in the urine samples was increased by 20% after 27-Gy radiation, which can be used as the specific markers of high-dose radiation damage.

Key words: metabolome, magnetic resonance spectroscopy, radiation injuries, alkaline phosphatase, superoxide dismutase

CLC Number:

R318

Wang Min, Pan Xiao-jing, Liu Bin, Zhang Hong. Radiation metabolomics of minimally invasive urine biomarkers for X-ray radiation exposure in mice[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(46): 8049-8055.

Figures/Tables 5

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