Bone morphogenetic protein-7 induces the expression of alkaline phosphatase in periosteal cells

doi:10.3969/j.issn.2095-4344.2013.33.003

Abstract

Abstract:

BACKGROUND: The reports on bone morphogenetic protein-7 as a stimulating factor to induce osteogenic are relatively rare.
OBJECTIVE: To study the expression of alkaline phosphatase of periosteal cells after induced by bone morphogenetic protein-7 in vitro.
METHODS: Periosteal cells were obtained from adult tibial periosteum, and then the periosteal cells were cultured by routine method in vitro. The cells were divided into experimental group and control group, and then cultured with bone morphogenetic protein-7 plus osteoblast culture adjuvants and simple osteoblast culture adjuvants, respectively. The phase contrast microscope was used to observe the morphology and ultrastructure of periosteal cells. Each group was observed at 7, 14 and 21 days, and three samples were observed at each time point. Alkaline phosphatase kit was used to detect the expression of osteoblast-specific markers alkaline phosphatase.
RESULTS AND CONCLUSION: After cultured for 7 days, the proliferation of periosteal cells in the experimental group and the control group was increased obviously, and the expression of alkaline phosphatase was detected but less. The cells were spindle in shape, while the expression of alkaline phosphatase in the experimental group was higher than that in the control group. After cultured for 14 days, the proliferation of periosteal cells in the experimental group and the control group was increased obviously, the cell morphology was changed from

spindle-shaped to wide spindle-shaped, and the expression of alkaline phosphatase in the experimental group was increased significantly when compared with the control group. After cultured for 21 days, the proliferation of periosteal cells was detected in the experimental group and the control group, and the proliferation in the experimental group was more significant than that in the control group, the cell morphology was wide spindle-shaped, and the number of alkaline phosphatase in the experimental group was higher than that in the control group. Statistical analysis showed that the positive rate of osteogenic markers alkaline phosphatase of bone morphogenetic protein-7 induced periosteal cells in the experimental group was higher than that in the control group (P < 0.01). It suggested that periosteal cells had the osteogenic and regeneration ability, the bone morphogenetic protein-7 could induce periosteal cells, promote the expression of alkaline phosphatase, and could induce the periosteal cells to transform into osteoblasts.

Key words: tissue construction, bone tissue construction, bone morphogenetic protein-7, periosteal cells, alkaline phosphatase, induction, osteoblasts, cell culture, provincial grants-supported paper

CLC Number:

R318

Liao Jia-cheng, Bei Kang-sheng, Lian Yin-chuan. Bone morphogenetic protein-7 induces the expression of alkaline phosphatase in periosteal cells[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.33.003.

Figures/Tables 7

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