Chemically extracted acellular allogenic nerve and accompanying peripheral vein for repair of facial nerve defects

doi:10.3969/j.issn.2095-4344.2013.18.014

Abstract

Abstract:

BACKGROUND: Studies have showed that repair of facial nerve defect in rabbit with chemically extracted acellular allogenic nerve can achieve good effect.
OBJECTIVE: To explore a more effective operation means to repair facial nerve defects on the basis of chemically extracted acellular allogenic nerve.
METHODS: New Zealand rabbits were randomly divided into the experimental group and the control group. The experimental group received chemically extracted acellular allogenic sural nerve transplantation and then received adventitial suture with accompanying peripheral vein to prepare the animal models of defects of buccal branches of facial nerve; the control group received the autologous facial nerve in situ anastomosis. The rabbits in the control group were treated with facial nerve cutting on the proximal and distal part of the same position that did not damage the normal anatomy relations between the cut nerve and the surrounding tissues, then the adventitia suture bridge was performed at the cutting site.
RESULTS AND CONCLUSION: At 3 months after operation, the animals of both groups survived well with basically symmetrical facial expression and normal moustache swinging, no formation of obvious scars and neuroma could be seen at the neural transplantation site. The microscope observation results showed that there were no significant differences in the conduction velocity of right facial nerve buccal branch, number of myelinated nerve fibers in the 5.0 mm segment of graft distal anastomosis and the count of target muscle motor endplate between two groups (P > 0.05). Repairing of rabbit facial nerve defects with chemically extracted acellular allogenic nerve and accompanying peripheral vein shows a postoperative result similar to that of autologous facial nerve in situ anastomosis.

Key words: organ transplantation, tissue transplantation, facial nerve, acellular, antigenicity, allogenic, nerve transplantation, vascularization, accompanying blood vessels, provincial grants-supported paper

CLC Number:

R318

Ma Hong-bin, Zhang Rong-ming. Chemically extracted acellular allogenic nerve and accompanying peripheral vein for repair of facial nerve defects[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(18): 3325-3332.

Figures/Tables 4

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