Anti-Staphylococcus aureus substance purification from Wu Gu Chong and its related antibacterial mechanisms

doi:10.3969/j.issn.2095-4344.2013.15.014

Abstract

Abstract:

BACKGROUND: The antibacterial substances purification from Wu Gu Chong is controversial, and the antibacterial mechanism is unclear.
OBJECTIVE: To isolate and purify antibacterial substances from Wu Gu Chong and to identify its related antibacterial mechanism.
METHODS: After ultra-filtration, the Turbidometric assay and Kirby-Bauer disk diffusion susceptibility test were used to examine the antibacterial activity of the fractions against Staphylococcus aureus. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to examine the effect of the extracts of maggot that was screened on the proliferation of Staphylococcus aureus and to determine the minimum inhibitory concentration. Electron microscopy was used to examine the ultra-structural changes of Staphylococcus aureus induced by antimicrobial substances of the extracts.
RESULTS AND CONCLUSION: The filtrate (Mr < 10 000) had the best antibacterial activity against Staphylococcus aureus (P < 0.05), which significantly inhibited the proliferation of Staphylococcus aureus. The minimum inhibitory concentration was 1 g/L. The antibacterial substances of maggot increased the permeability and destroyed the cell membrane of Staphylococcus aureus. The anti-Staphylococcus aureus substances from Wu Gu Chong was less than 10 000, and its related mechanisms of anti-bacterial effect was a disruption of the outer and cytoplasmic membranes leading to lysis of the target cells.

Key words: tissue construction, cytology experiment in tissue construction, Wu Gu Chong, antimicrobial substances, ultrafiltration, antibacterial mechanism, Staphylococcus aureus, antibacterial activity, membrane permeability, minimum inhibitory concentration, separation, purification, National Natural Science Foundation of China

CLC Number:

R318

Zhang Zhen, Hong Liang, Wang Shou-yu, Lü De-cheng. Anti-Staphylococcus aureus substance purification from Wu Gu Chong and its related antibacterial mechanisms[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.15.014.

Figures/Tables 6

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