Primary culture of human hypertrophic scar fibroblasts and its biological behavior

doi:10.3969/j.issn.2095-4344.2013.07.007

Abstract

Abstract:

BACKGROUND: Hypertrophic scar is inevitable in the process of wound healing, but the hypertrophic scar tissue can result in many adverse effects.
OBJECTIVE: To establish a reliable method to culture human hypertrophic scar fibroblasts in vitro.
METHODS: Human hypertrophic scar fibroblasts were cultured primarily in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum using tissue adherent method and digestion method. Fibroblasts were incubated at 37℃in a humidified atmosphere of 5% carbon dioxide. Cellular morphologies were observed, the growth curve was drawn, and vimentin expression wass detected.
RESULTS AND CONCLUSION: (1) After cultured with tissue adherent method, human hypertrophic scar fibroblasts were cultured successfully, first passaged within 20-40 days and then continued to passage every 7-10 days. The cells were long spindle-shaped, and vimentin was positive. (2) Human hypertrophic scar fibroblasts, cultured using digestion method, were cultured successfully and confluent at 15-20 days. The cells were long spindle-shaped, and vimentin was positive. These data have confirmed that both tissue adherent method and digestion method are successful in the primary culture of human hypertrophic scar fibroblasts.

Key words: tissue construction, skin tissue construction, human hypertrophic scar, fibroblasts, primary culture, cell culture, adherent method, digestion, vimentin, biological behavior, cell division, mitotic index, tissue construction photographs-containing paper

CLC Number:

R318

Du Qi-cui, Xiao Wen-lin, Sun Gui-lan, Ma Xiu-xing, Yao Ru-yong. Primary culture of human hypertrophic scar fibroblasts and its biological behavior[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(7): 1174-1179.

Figures/Tables 11

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