Effects of self-made cryopreservation solution on oxygen free radicals in cryopreserved rat liver

doi:10.3969/j.issn.2095-4344.2013.05.012

Abstract

Abstract:

BACKGROUND: During liver transplantation, cryopreservation and ischemia can lead to production of oxygen free radicals by the liver which damages liver tissue.
OBJECTIVE: To study the effects of self-made cryopreservation solution on oxygen free radicals in cryopreserved rat liver, and to compare with “gold standard” organ preservation solution-UW solution.
METHODS: Sixteen 9-week-old Sprague Dawley rats were randomly divided into two groups: experimental group and control group, 8 rats in each group. Sprague Dawley rat liver perfusion models were established, and the livers in each group were lavaged with self-made organ preservation solution and UW preservation solution, then removed the liver and placed into 4℃preservation solution. The superoxide dismutase, nitric oxide synthase, total antioxidant capacity activity and the malondialdehyde content were detected at 24, 48 and 72 hours after preservation.
RESULTS AND CONCLUSION: There were no significant differences in superoxide dismutase, nitric oxide synthase, total antioxidant capacity activity and the malondialdehyde content in rat liver tissue cryopreserved in the self-made organ preservation solution versus UW solution (P > 0.05). These findings suggest that self-made organ preservation solution can reduce the damage of oxygen free radical to the livers of rats after ischemia-reperfusion and the effect is equivalent to UW solution.

Key words: organ transplantation, liver transplantation, organ preservation solution, self-made organ preservation solution, UW solution, cryopreservation, liver, transplantation, oxygen free radicals, provincial grants-supported paper

CLC Number:

R318

Wang Lu-jia, Sun Wen, Zhang Jin-ping, Wang Chao, Zhao Xiao-yu, Zhu Ling-ying, Ma Jing-ru, . Effects of self-made cryopreservation solution on oxygen free radicals in cryopreserved rat liver[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(5): 837-842.

Figures/Tables 4

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