Abstract:

BACKGROUND: Defatted and deproteinsed cancellous bone allograft has good biological properties. It has a three- dimensional structure that is the same as the recipient’s, stable mechanical properties, weak rejection and excellent cytocompatibility.
OBJECTIVE: To prepare defatted and deproteinsed cancellous bone allograft scaffold materials by physiochemical methods, and to analyze physical and chemical characteristics.
METHODS: Healthy 6-month-old New Zealand white rabbits were chosen, and 20 iliac bones were dissected into 1.0 cm×0.8 cm×0.1 cm bone. Bone scaffold material was prepared by defatting, deproteinization, as well as low and depths freezing temperatures. Its physical and chemical characteristics were tested, and the adhesion rate of the scaffold material and bone marrow stromal stem cells was detected. The scaffold was implanted into the same animal species, and histocompatibility and immunoreaction were observed.
RESULTS AND CONCLUSION: The scaffold materials reserved the mesh pore structures of natural bone tissues with the pore ratio of (80.23±5.65)%. Maximum aperture was (318.112±17.51) μm, and minimum aperture was (209.37±11.33) μm. Bone marrow stromal stem cells could not only adhere to the scaffold, but also divide and proliferate on the scaffold. After implantation for 6 weeks, there were no obvious inflammation and rejection around the scaffold interface and a small amount of bone-like tissues formed. These results suggest that the deffatted and deproteinsed cancellous bone scaffold has the appropriate three-dimensional porous structure, and adhesion rate of seed cells is high, besides, it has good biocompatibility and cell-material interface role; at the same time there is a certain osteogenesis function.