Abstract:

BACKGROUND: The similarities and differences between opaque cornea of B6-Co mutant mice and normal cornea of normal B6 mice at the proteomic level have been studied, which can help to illuminate the mechanisms of human corneal opacity and develop new ways for studying gene function using the phenotype-driven approach.
OBJECTIVE: To compare and analyze the similarities and differences of cornea proteomics between B6 mice and B6-Co mutant mice using two-dimensional gel electrophoresis method.
METHODS: After the corneal tissue protein of B6 mice and B6-Co mutant mice was extracted respectively, the samples acquired were performed two-dimensional gel electrophoresis and gel staining. And then, the electropherograms were analyzed for comparing the similarities and differences of the protein between normal and opaque corneal tissues.
RESULTS AND CONCLUSION: The extract of the corneal tissue protein, basic method and condition of two-dimensional gel electrophoresis were established successfully. The gel electropherogram was clear and the result showed that 13 protein spots were significantly downregulated and six protein spots were significantly upregulated in opaque corneal protein of B6-Co mutant mice. There was a significant difference in cornea proteomics between B6 mice and B6-Co mutant mice. These results suggest that gene mutation can lead to the gene up-, and down-regulation or silencing in related signal pathways.