Abstract:

BACKGROUND: Stromal cell derived factor-1(SDF-1)/chemokine receptor 4 (CXCR4) signal pathway plays a key role in the pathogenesis of osteoarthritis.
OBJECTIVE: To explore the effect of SDF-1/CXCR4 signal pathway on the expression of type Ⅱ collagen and aggrecan mRNA of the synthesis in human articular cartilage after blocking the SDF-1/CXCR4 signal pathway with AMD3100.
METHODS: The cartilage blocks from osteoarthritis patients who had total knee replacement and normal cartilage blocks from patients who had traumatic amputation were collected and divided into three groups: experimental group, experimental control group and blank control group. The three groups were cultured in the nutrient solution containing of SDF-1 and AMD3100, SDF-1 and MAB310, as well as SDF-1 only, respectively.
RESULTS AND CONCLUSION: The expressions of type Ⅱ collagen and aggrecan mRNA, as well as type Ⅱ collagen content in the experimental group was higher than those in the experimental control group and blank control group (P < 0.05). These results suggest that SDF-1 can induce the degradation of type Ⅱ collagen and aggrecan thruogh the SDF-1/CXCR4 signaling pathway. Besides, AMD3100 can block SDF-1/CXCR4 signal pathway and reduce the degradation of type II collagen and aggrecan, and therefore slow down the degeneration of articular cartilage. But AMD3100 cannot recover type II collagen and aggrecan in osteoarthritis cartilage to normal levels