Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (46): 8571-8574.doi: 10.3969/j.issn.2095-4344.2012.46.005

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Explant culture and identification of SD rat’s osteoblasts

Li Juan1, 2, Shen Guang-hao1, Cheng Guang2, Luo Er-ping1, Li Fei-jiang1, Jing Da1, Fei Zhou2, Chen Xiao-yan2   

  1. 1Health Equipment and Metrology Staff Room, Department of Biomedical Engineering, the Fourth Military Medical University of Chinese PLA, Xi’an 710032, Shaanxi Province, China
    2Military Institute of Clinical Neuroscience, Xijing Hospital, Fourth Military Medical University of Chinese PLA, Xi’an 710032, Shaanxi Province, China
  • Received:2012-02-19 Revised:2012-04-09 Online:2012-11-11 Published:2013-03-16
  • Contact: Shen Guang-hao, Doctor, Associate professor, Master’s supervisor, Health Equipment and Metrology Staff Room, Department of Biomedical Engineering, the Fourth Military Medical University of Chinese PLA, Xi’an 710032, Shaanxi Province, China mmem2002@fmmu.edu.cn Cheng Guang, Doctor, Associate professor, Master’s supervisor, Military Institute of Clinical Neuroscience, Xijing Hospital, Fourth Military Medical University of Chinese PLA, Xi’an 710032, Shaanxi Province, China
  • About author:Li Juan★, Studying for master’s degree, Health Equipment and Metrology Staff Room, Department of Biomedical Engineering, the Fourth Military Medical University of Chinese PLA, Xi’an 710032, Shaanxi Province, China; Military Institute of Clinical Neuroscience, Xijing Hospital, Fourth Military Medical University of Chinese PLA, Xi’an 710032, Shaanxi Province, China juanli834@126.com

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Abstract:

BACKGROUND: It is difficult to acquire sufficient osteoblasts with high purity. Therefore, it is imperative to master simple and fast extraction osteoblast method for culture and identification.
OBJECTIVE: To culture in vitro and identify SD rat’s osteoblasts with explant culture.
METHODS: The newborn (< 24 hours) SD rats were executed, and the skulls were taken out. Excess tissues around the skull were removed. The picked skull was cut off into 1 mm3 pieces. The osteoblasts were cultured by conventional method and tissue explant (modified method) as well as identified with morphology, alkaline phosphatase assay and Von Kossa staining methods.
RESULTS AND CONCLUSION: The osteoblasts cultured by tissue explant had typical morphological characteristics; alkaline phosphatase was positive staining, and mineralization nodules formed after Von Kossa staining. Besides, the osteoblasts had unitary cell component which was typical, and the culture time was short, what’s more, the quantity, purity and density were coincident. These findings suggest that the osteoblast lineage acquired is stable, which provides a good plate for in vitro experiments.

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