Abstract:

BACKGROUND: How to protect the non-heart-beating donor lung in the warm ischemia period in order to reduce the primary graft dusfunction after transplantation is the most important issue to all the researchers and clinicians.
OBJECTIVE: To assess the effect of part liquid ventilation in the protection of non-heart-beating donor lung from pathology.
METHODS: Thirty-six health SD rats were randomly divided into three groups. Homemade 16G deep vein needle lien was used for endotracheal intubation and connected to a respirator machine with mechanical ventilation. The KACL solution was injected into the jugular and lead to the rat sudden death, the multi-channel physiological detector was used to continuous record the blood pressure, the model was successful when the blood pressure was 0 mmHg. After the models were established, the models in the normal mechanical ventilation group were preformed with mechanical ventilation for 2 hours; the models in the saline group and the perfluoro carbon group were given oxygen ventilation for 5 minutes, then injected with the equivalent of the functional residual capacity of the oxygen salt and high oxygen perfluoro carbons (10 mL/kg) by endotracheal intubation, and mechanical ventilation for 2 hours.
RESULTS AND CONCLUSION: General observation showed that the lung of saline group was swelling, the sheet surface of lung tissue was bleeding, the lung was dark red, airway edema with bloody fluid; the lung of oxygen group was less swelling and less bleeding points of lung surface; in the perfluoro carbon group, the lung tissue inflammatory cell infiltration and edema was reduced. Light microscope observation of saline group and oxygen group showed that there was diffuse alveolar and interstitial hyperemia and edema, a large number of inflammatory cell infiltration could be seen in the bronchial wall and capillaries surrounding, the alveolar was over-expansion and the alveolar wall was ruptured; the lung tissue structure in the perfluoro carbon group was more complete and no significant damage. The results show that partial liquid ventilation can play a very good role in the protection of the non-heart-beating donor lungs.