Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (31): 5701-5705.doi: 10.3969/j.issn.2095-4344.2012.31.001

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Inhibition of acute allograft rejection in a rat liver transplantation model by adenovirus associated plasmid transferred with indoleamine 2,3-dioxygenase

Liu Xiao-long1, Han Lu-zhe1, Ren Xin1, Xiao Gang1, Jin Yi-yi1, Chen Yong-bing2, Liu Li-xin1   

  1. 1Department of General Surgery, Third Affiliated Hospital of Southern Medical University, Guangzhou 510630, Guangdong Province, China;
    2Department of General Surgery, General Hospital of Beijing Military Command, Beijing 100007, China
  • Received:2012-01-27 Revised:2012-03-07 Online:2012-07-29 Published:2012-07-29
  • Contact: Liu Li-xin, Doctor, Chief physician, Department of General Surgery, Third Affiliated Hospital of Southern Medical University, Guangzhou 510630, Guangdong Province, China
  • About author:Liu Xiao-long☆, Doctor, Attending physician, Department of General Surgery, Third Affiliated Hospital of Southern Medical University, Guangzhou 510630, Guangdong Province, China g_xlliu@126.com

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Abstract:

BACKGROUND: Indoleamine 2,3-dioxygenase (IDO) expressing in immune privilege site can induces the T cell immune tolerance.
OBJECTIVE: To study the inhibitory effect of adenovirus associated plasmid vector medicated IDO on acute rejection following rat liver allograft.
METHODS: IDO and pWAV2 were transferred into a new vector pWCM-IDO. Forty-eight PVG/DA liver transplant models were randomly devided into three groups: normal saline group, pWAV2 group and pWCM-IDO group. Models in the three groups were performed with intraperitoneal injection of 1 mL normal saline, pWAV2 and pWCM-IDO, respectively.
RESULTS AND CONCLUSION: pWCM-IDO could be successfully transfected into the PVG donor liver. The survival time and the IDO expression level in the PWCM-IDO group were significantly higher than those in the normal saline group and pWAV2 group (P < 0.05-0.01). At 4 days after transplantation, the acute rejection degree in the PWCM-IDO group was significantly weaker than that in the other two groups. IDO recombinant plasmid could be successfully transfected into rat liver to relieve the acute rejection following liver allograft. But it difficult to sustain the effective expression, and the inhibitory effect on immune rejection was limited.

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