Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (27): 5108-5112.doi: 10.3969/j.issn.2095-4344.2012.27.032

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Identification of neural stem cells

Zhang Jun-hu, Wang Jian-jiao, Zheng Yong-ri   

  1. Department of Neurosurgery, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
  • Received:2011-11-02 Revised:2011-11-30 Online:2012-07-01 Published:2013-11-01
  • Contact: Zheng Yong-ri, Chief physician, Master’s supervisor, Department of Neurosurgery, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
  • About author:Zhang Jun-hu★, Studying for master’s degree, Department of Neurosurgery, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China zjhgx6868@163.com

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Abstract:

BACKGROUND: Due to the less number of neural stem cells, no specific morphology and lack of surface antigen, the identification and purify of the neural stem cells are difficult.
OBJECTIVE: To summarize the main method to identify the neural stem cells.
METHODS: The PubMed database and Wanfang database form 1990 to 2011 were used to search the articles on the characteristics of neural stem cells, main identification method and application prospects.
RESULTS AND CONCLUSION: At present, the main method to identify the neural stem cells was in vivo dye identification method, surface marker identification method, molecular biology identification method and nuclide notation. The markers included Nestin, vimentin, Musashi1, Musashi2, CD133 (AC133), sialic acid-neural cell adhesion molecule, Lewis X LeX/SSEA-1, p75 neurotrophin receptor and epidermal growth factor. The surface marker identification method, molecular biology identification method and the combination of two methods were the main methods to identify the neural stem cells. It is a possible research tools to obtain breakthrough.

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