Association between vitamin D receptor gene polymorphism and semi-quantitative classification of thoracolumbar osteoporotic vertebral compression fractures

doi:10.3969/j.issn.2095-4344.1989

Abstract

Abstract:

BACKGROUND: Vitamin D receptor gene plays a key role in regulating calcium and phosphorus metabolism and bone metabolism. There are no reports on its association with osteoporotic vertebral compression fractures.
OBJECTIVE: To investigate the relationship between vitamin D receptor gene polymorphism and semi-quantitative classification of thoracolumbar osteoporotic vertebral compression fractures by restriction fragment length polymorphism polymerase chain reaction.
METHODS: The study was in accordance with the ethical requirements of the First Affiliated Hospital of Guangxi University of Chinese Medicine, and all participants signed the informed consents. Sixty patients with thoracolumbar osteoporotic vertebral compression fractures were randomly selected. The standard lateral X-ray films of the whole spine were taken. The Genant semi-quantitative method was used to classify the fracture types. The serum of peripheral blood was collected, and the genotype of vitamin D receptor polymorphism was identified by DNA amplification, specific enzyme digestion and gel electrophoresis imaging. The correlation between the polymorphisms of the vitamin D receptor and the thoracolumbar osteoporotic vertebral compression fractures classification was analyzed by statistical methods.
RESULTS AND CONCLUSION: (1) Vitamin D receptor gene BB type was detected in 0 case, Bb type in 28 cases and BB type in 32 cases.
(2) Type II (moderate compression) and type III (severe compression) Bb vertebral fractures accounted for 87%, compared with Bb group accounted for 32%, the difference was significant (χ2=57.236, P < 0.01). (3) These results indicate that different genotypes of vitamin D receptor in thoracolumbar osteoporotic vertebral compression fractures patients are correlated with fracture types. The risk of moderate to severe compressive vertebral fractures in patients with Bb genotype is higher than that in patients with bb genotype.

Key words: vitamin D receptor, genotype, gene polymorphism, vertebral fracture, compressive fracture, osteoporosis, fracture classification, quantitative classification

CLC Number:

Lu Qiuping1, Gui Yuchang2, Xu Jianwen2, Qin Haibiao3, Rao Yuansen1. Association between vitamin D receptor gene polymorphism and semi-quantitative classification of thoracolumbar osteoporotic vertebral compression fractures[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(35): 5577-5581.

Figures/Tables 2

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