Chinese Journal of Tissue Engineering Research ›› 2020, Vol. 24 ›› Issue (1): 51-58.doi: 10.3969/j.issn.2095-4344.1861
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Ruan Guangping1, 2, 3, Yao Xiang1, 2, 3, Cai Xuemin1, 2, 3, Li Zian1, 2, 3, Pang Rongqing1, 2, 3, Pan Xinghua1, 2, 3
Received:
2019-03-13
Revised:
2019-03-21
Accepted:
2019-04-19
Online:
2020-01-08
Published:
2019-12-11
Contact:
Pan Xinghua, MD, Chief physician, The Cell Biological Therapy Center of 920th Hospital of Joint Logistics Support Force of PLA, Kunming 650032, Yunnan Province, China; The Integrated Engineering Laboratory of Cell Biological Medicine of State and Regions, Kunming 650032, Yunnan Province, China; The Transfer Medicine Key Laboratory of Cell Therapy Technology of Yunan Province, Kunming 650032, Yunnan Province, China
About author:
Ruan Guangping, MD, Associate chief physician, The Cell Biological Therapy Center of 920th Hospital of Joint Logistics Support Force of PLA, Kunming 650032, Yunnan Province, China; The Integrated Engineering Laboratory of Cell Biological Medicine of State and Regions, Kunming 650032, Yunnan Province, China; The Transfer Medicine Key Laboratory of Cell Therapy Technology of Yunan Province, Kunming 650032, Yunnan Province, China
Supported by:
CLC Number:
Ruan Guangping, Yao Xiang, Cai Xuemin, Li Zian, Pang Rongqing, Pan Xinghua. Allogeneic umbilical cord mesenchymal stem cell transplantation for metabolic syndrome in a tree shrew model[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(1): 51-58.
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2.2 代谢综合征模型建立及脐带间充质干细胞治疗结果 2.2.1 一般情况 模型组40只树鼩,最终有32只成模。模型组树鼩与正常对照组比较,出现毛色灰暗、尾部溃疡坏死、多饮、腹泻、反应迟钝、活动度差等症状;正常对照组树鼩活跃、反应灵敏,无上述症状;而治疗组树鼩在治疗后2周尾部溃疡开始愈合,腹泻减少,活动度增加,反应较模型组树鼩灵敏。 2.2.2 空腹血糖水平变化 高糖高胆固醇高盐饲料饲喂4周,模型组树鼩空腹血糖相比对照组已有升高;8周时,模型组树鼩空腹血糖均值已达10 mmol/L以上。注射链脲佐菌素后空腹血糖达到11.1 mmol/L以上,并维持在一个较高水平[模型组:(25.50±4.50) mmol/L;正常对照组:(4.19± 0.77) mmol/L,P < 0.01]。脐带间充质干细胞治疗后树鼩空腹血糖较模型组及治疗前明显下降,并随时间延长呈下降趋势,但未能降至正常对照组正常水平,见图2。 "
2.2.3 空腹胰岛素水平变化 从高糖高胆固醇高盐饲料饲喂开始,模型组空腹胰岛素水平就呈上升趋势,高饲8周时,模型组树鼩胰岛素水平达到高峰,注射链脲佐菌素后,进一步破坏了胰岛β细胞,使细胞出现变性坏死,其分泌功能开始失代偿,胰岛素分泌量开始下降至低于正常对照组水平[模型组:(0.96±0.11) μIU/L;正常对照组:(2.37± 1.92) μIU/L,P < 0.01],见图3A。脐带间充质干细胞治疗后4周,治疗组树鼩胰岛素分泌量明显增加[治疗组:(1.34±0.33) μIU/L;模型组:(0.85±0.10) μIU/L,P < 0.05],差异有显著性意义,见图3B。 2.2.4 胰岛素抵抗指数变化 高糖高胆固醇高盐饲料饲喂8周时,模型组树鼩已表现出明显的胰岛素抵抗[模型组:(4.50±0.51)×10-3,正常对照组:(0.40±0.10)×10-3,P < 0.01],注射链脲佐菌素后,由于持续的高水平空腹血糖使得胰岛功能开始失代偿,胰岛素水平下降,胰岛素抵抗指数也较前降低,但仍明显高于正常对照组[模型组:(1.01±0.02)×10-3,正常对照组:(0.44±0.07)×10-3,P < 0.01],见图4A。治疗后4周,治疗组树鼩胰岛素抵抗指数由(1.10±0.10)×10-3降至(0.74±0.02)×10-3,较治疗前降低,差异有显著性意义(P < 0.05),见图4B。 "
2.2.6 血浆三酰甘油、总胆固醇及低密度脂蛋白胆固醇浓度变化 高糖高胆固醇高盐饲料饲喂1周,模型组树鼩的三酰甘油、总胆固醇及低密度脂蛋白胆固醇都开始升高,第4周时明显升高,差异有显著性意义;此后整个造模过程中呈现出不稳定的升高趋势,三酰甘油由(0.63±0.17) mmol/L上升至(2.11±0.23) mmol/L,总胆固醇由(2.31± 0.29) mmol/L上升至(5.57±0.94) mmol/L;低密度脂蛋白胆固醇从高饲1周开始即一直保持明显的升高趋势,由(0.20±0.14) mmol/L上升至(0.77±0.18) mmol/L;正常对照组树鼩血脂在造模过程中并无差异性变化,见图6A,C,E。以上表明模型组树鼩血脂代谢紊乱。脐带间充质干细胞治疗后血脂明显降低,三酰甘油由(1.49±0.23) mmol/L降至(0.71±0.09) mmol/L,总胆固醇由(4.97±0.48) mmol/L降至(2.51±0.73) mmol/L,低密度脂蛋白胆固醇由(0.79± 0.16) mmol/L降至(0.51±0.17) mmol/L,与治疗前及模型组相比差异有显著性意义(P < 0.01),见图6B,D,F。"
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