Inhibitory effect of complex polysaccharides on ultraviolet-induced photoaging of skin cells

doi:10.3969/j.issn.2095-4344.1804

Abstract

Abstract:

BACKGROUND: In addition to aging, skin photoaging is directly related to sunlight exposure. Photoaging refers to skin aging caused by sunlight. In recent years, increasing evidence has shown that polysaccharides can certainly inhibit skin photoaging, as they have antioxidant, moisturizing, anti-radiation functions.
OBJECTIVE: To explore whether complex polysaccharides are superior to single polysaccharides to inhibit photoaging of skin cells.
METHODS: Irradiation by ultraviolet light was conducted to damage human skin fibroblasts (HSF) and human immortalized keratinocytes (HaCaT). Both kinds of cells were purchased from the Cell Bank of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. On the basis of this, MTT assay and biochemical kits were used to detect the survival rate, superoxide dismutase, malondialdehyde and hydroxyproline levels in HSF and HaCaT photoaging cell models after treated with four kinds of single polysaccharides (polysaccharide from Dendrobium officinale, polysaccharide from Phaeophyta, polysaccharide from Ganoderma Lucidum and polysaccharide from Dictyophora indusiata) and four kinds of polysaccharide mixtures (i.e., complex polysaccharides) at the same concentration.
RESULTS AND CONCLUSION: (1) Compared with the model group, the interventional results of single-component polysaccharides on HSF and HaCaT photoaging cells showed a significant improvement in some but not all biochemical indicators (P < 0.01 or P < 0.05). Four kinds of single polysaccharides had similar protective effects on photoaging cell models. (2) Compared with the model group, complex polysaccharides at 50 mg/L could significantly increase the cell survival rate and levels of superoxide dismutase and hydroxyproline, and decrease malondialdehyde level in HSF and HaCaT photoaging cell models (P < 0.01 or P < 0.05). To conclude, in the anti-ultraviolet-induced HSF and HaCaT photoaging experiments, the complex polysaccharides (polysaccharide from Dendrobium officinale+polysaccharide from Phaeophyta+polysaccharide from Ganoderma Lucidum+polysaccharide from Dictyophora indusiata) were better than the single polysaccharide of each component at the same intervention concentration.

Key words: polysaccharide from Dendrobium officinale, polysaccharide from Phaeophyta, polysaccharide from Ganoderma Lucidum, polysaccharide from Dictyophora indusiata, keratinocyte, fibroblast, superoxide dismutase, hydroxyproline, malondialdehyde

CLC Number:

Yang Kaiye, Wang Liping, Wang Bingxin, Lu Yingyu, Feng Jiao, Wu Meiyin, Shi Wan, Hou Shaozhen. Inhibitory effect of complex polysaccharides on ultraviolet-induced photoaging of skin cells[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(29): 4707-4713.

Figures/Tables 6

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