Embryonic development of C-type natriuretic peptide-pretreated mouse oocytes

doi:10.3969/j.issn.2095-4344.1707

Abstract

Abstract:

BACKGROUND: C-type natriuretic peptides as physiological inhibitors can inhibit meiosis in mouse oocytes, but whether it promotes the developmental ability of the obtained embryos or not is still unclear.
OBJECTIVE: To study the effect of C-type natriuretic peptide pretreatment on the quality of mouse embryo.
METHODS: Mouse oocytes were pretreated with C-type natriuretic peptide for 8 hours, and then undertook in vitro maturation for 24 hours. The mature oocytes were fertilized in vitro to obtain embryos (experimental group). The embryos obtained from mouse oocytes normally cultured in vitro for 24 hours were used as the control group. The study protocol in the experimental group was approved by the Animal Experimental Ethics Committee of the Wulanchabu Medical College, with the approval No. WYZLL[2016]1601.
RESULTS AND CONCLUSION: The average number of blastocysts in the experimental group was significantly higher than that in the control group (P < 0.05). The number of mitochondrial copies in these two groups increased gradually with the development of mouse embryo, but increased faster in the experimental group than the control group (P < 0.05). The reactive oxygen species level in the experimental group was significantly lower than that in the control group (P < 0.05), and the glutathione level of embryonic cells in the experimental group was significantly higher than that in the control group (P < 0.05). To conclude, C-type natriuretic peptide pretreatment has significantly improved the developmental capacity of mouse embryos.

Key words: C-type natriuretic peptide, two-stage culture system, mouse embryo, developmental ability, number of blastocysts, mitochondrial DNA copy number, glutathione, reactive oxygen species

CLC Number:

R456
R394

Zhang Chunqiang, Zhang Tong, Zhang Guiying, Liu Yu, Wang Shuying, Liu Gaiping, Zhang Jiaxin. Embryonic development of C-type natriuretic peptide-pretreated mouse oocytes[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(17): 2722-2727.

Figures/Tables 6

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