Tranosseous suture bridge technique versus modified Mason-Allen technique repairs unilateral rotator cuff tears in rabbits

doi:10.3969/j.issn.2095-4344.1485

Abstract

Abstract:

BACKGROUND: The modified Mason-Allen technique is commonly used for repairing rotator cuff tears in small animals, while the suture bridge technique is rarely reported.
OBJECTIVE: To explore a new method for repairing rotator cuff injury in small animals.
METHODS: The study was approved by the Experimental Animal Ethics Committee of North Sichuan Medical College. Forty-eight male New Zealand white rabbits were used to make rotator cuff injury models and randomly divided into control (repaired by Mason-Allen technique) and experimental (repaired by tranosseous suture bridge technique) groups. Sixteen animals were sacrificed at 4, 8, and 12 weeks, respectively, and the proximal humerus specimens were used for gross observation, histological examination, and modified maturity of tendon-bone healing score.
RESULTS AND CONCLUSION: (1) Gross observation: there were two cases of fixation failure in the control group. (2) Histological examination: at 12 weeks after modeling, in the experimental group, the chondrocytes arranged regularly, and fibrocartilage arranged densely and regularly, which similar with columnar structure. The tendon-bone interface in the control group revealed scar healing mainly, and few chondrocytes and fibrocartilage that arranged in disorder were observed. (3) At 4, 8 and 12 weeks, the scores of tendon-bone healing in the experimental group were significantly better than those in the control group (P < 0.01). (4) These results indicate that compared with modified Mason-Allen technique, the transosseous suture bridge technique possesses higher maturity of tendon-bone healing score and lower fixation failure rate, and improves the rotator cuff tendon-bone healing rate, which is a better method to repair rotator cuff injury in animal models.

Key words: rotator cuff tear, tendon-bone healing, transosseous, suture bridge, animal modeling, rabbits

CLC Number:

Kou Jinghao1, 2, Pu Jingsong1, Jiang Cheng1, Liu Tao2, Lei Xiaoyu2 . Tranosseous suture bridge technique versus modified Mason-Allen technique repairs unilateral rotator cuff tears in rabbits [J]. Chinese Journal of Tissue Engineering Research, 2019, 23(35): 5605-5610.

Figures/Tables 2

2.1 实验动物数量分析实验中48只实验动物未出现死亡、感染等情况，均达到取材标准。 2.2 大体标本观察 2组实验动物术区均出现皮下组织与三角肌不同程度粘连(图1F)。术后4周，2组实验动物重建部位为白色纤维样物质包绕，缝线均张力良好，无肌腱切割。术后8周及12周，对照组中各出现1例重建部位再撕裂样本，即固定失效，实验组无固定失效样本。 2.3 组织学观察 2.3.1 对照组术后4周：腱骨界面连接不紧密，由新生的肉芽组织填充，但填充并不完全，可见新生血管及成纤维细胞，及较多的炎性细胞；界面内未见软骨细胞生长，新生的胶原纤维较少(见图2A)。术后8周：腱骨界面已完全填充，成纤维细胞较多，其中仍可见少量的血管，胶原纤维已生长，但排列紊乱，骨端少量未分化成熟的空泡状软骨细胞(见图2B)。术后12周：腱骨连续性已趋向于完整，界面内以瘢痕组织为主，软骨细胞和胶原纤维较少且排列紊乱(见图2C)。修复失败的模型中，腱骨之间无连续性，肌腱端已脂肪化(见图2D)。 2.3.2 实验组术后4周：肌腱与骨之间无明显缝隙，腱骨之间由新生的肉芽组织完全填充，含有较多的新生血管及成纤维细胞，少量未成熟的软骨细胞生长，胶原纤维排列紊乱(见图2E)。术后第8周：腱骨界面已完全模糊，肌腱与骨之间的组织明显增厚，界面内软骨细胞生长明显，排列仍较紊乱，依然处于分化未成熟阶段，胶原纤维排列较4周更为致密、整齐(见图2F)。术后12周：腱骨已趋于完全愈合，腱骨界面连续性完好，无血管生长，软骨细胞分化成熟，胶原纤维排列较整齐致密，趋于正常的柱状结构(见图2G)。 2.4 统计学分析结果在术后相同的4，8，12周3个时间点，实验组的腱骨愈合成熟度评分均高于对照组(P < 0.01)，见表2。 "

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