Chinese Journal of Tissue Engineering Research ›› 2014, Vol. 18 ›› Issue (51): 8297-8300.doi: 10.3969/j.issn.2095-4344.2014.51.018

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Isolation, culture and identification of rabbit tendon cells

Wang Yu-cong1, Zhang Qian-fa2   

  1. 1Department of Orthopedics, Ninth People’s Hospital of Ningbo City, Ningbo 315020, Zhejiang Province, China; 2Department of Orthopedics, Baoshan Branch, First People’s Hospital of Shanghai, Shanghai 200400, China
  • Online:2014-12-10 Published:2014-12-10
  • Contact: Zhang Qian-fa, Chief physician, Department of Orthopedics, Baoshan Branch, First People’s Hospital of Shanghai, Shanghai 200400, China
  • About author:Wang Yu-cong, Master, Department of Orthopedics, Ninth People’s Hospital of Ningbo City, Ningbo 315020, Zhejiang Province, China

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Abstract:

BACKGROUND: Tendon cells are characterized by high differentiation potentials, slow proliferation rate, and even lost proliferation capacity after passage in vitro. Therefore it is necessary to establish the ideal isolation and culture patterns of tendon cells in vitro.

 
OBJECTIVE: To investigate the isolation, culture and identification of tendon cells.
METHODS: The flexor tendon of New Zealand fetal rabbits were cut under sterile conditions, the peritenon of flexor tendon was removed by microsurgical technique. Tendon cells were isolated with Henderson-step enzymatic digestion method and cultured in a complete medium consisting of DMEM/F12 and 20% fetal bovine serum for primary culture and passage.
RESULTS AND CONCLUSION: The tendon cells with high purity can be successfully isolated by different enzymatic digestion methods, and the cultured cells well proliferated and passaged in vitro. The immunohistochemical staining showed that, passage 2 cells were positive for collagen type I antibody, but negative for collagen type III antibody. These evidences confirmed that the cultured cells are tendon cells. Tendon cells can be isolated, proliferated and passaged in vitro.


中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程


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Key words: cell, cultured, immunohistochemistry, rabbits, tendon

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