Effects of Buyang Huanwu decoction on the expression of collagen type I and IV after spinal cord injury

doi:10.3969/j.issn.2095-4344.1459

Abstract

Abstract:

BACKGROUND: Collagen type I and IV are shown to be beneficial for limb locomotion recovery after spinal cord injury.
OBJECTIVE: To explore the mechanism of Buyang Huanwu decoction on the rehabilitation of limb locomotion of rats after spinal cord injury by observing the changes of limb locomotion and expression levels of collagen type I and IV.
METHODS: The study was approved by the Experimental Animal Ethics Committee of Fujian University of Traditional Chinese Medicine, approval number: SYXK2013-005. Fifty-four Sprague-Dawley rats were divided into three groups, sham, model and Buyang Huanwu decoction groups (n=18/group). Rats in the sham group underwent the laminectomy and rats in the other groups were used for establishing the spinal cord injury model by NYU impactor. The rats in the Buyang Huanwu decoction group, and sham and model groups were administrated 1 mL of Buyang Huanwu decoction and normal saline via gavage, respectively, at 1 day post-injury. Basso, Beattie, and Bresnahan limb locomotion scores were evaluated at 1, 3, 5 and 7 days after injury. At 7 days after injury, samples were removed for Nissl staining to observe the morphology of spinal cord neurons and the expression levels of collagen type I and IV were detected by immunohistochemistry. The ultrastructures of medullary sheath were observed under transmission electron microscope. The expression levels of collagen type I and IV proteins were detected by western blot assay.
RESULTS AND CONCLUSION: (1) The Basso, Beattie, and Bresnahan limb locomotion scores from the fifth day after modeling in the Buyang Huanwu decoction group were significantly higher than those in the model group (P < 0.05). (2) Nissl staining showed that in the Buyang Huanwu decoction group, the structure of neurons was complete, cell edema was slight, hemostasis area was small, and the recovery of neurons was better than that in the model group. (3) Transmission electron microscope showed that the structure of myelin sheath in the Buyang Huanwu decoction group was better than that in the model group, and with slight edema, which were similar with the Nissl staining results. (4) Immunohistochemistry results showed that the expression levels of collagen type I and IV in the Buyang Huanwu decoction group were significantly higher than those in the model group (P < 0.05). (5) Western blot assay results showed that expressions of collagen type I and IV proteins in the Buyang Huanwu decoction group were significantly higher than those in the model group (P < 0.05). (6) In summary, Buyang Huanwu decoction can promote the rehabilitation of limb locomotion of rats after spinal cord injury, possibly by up-regulating the expressions of collagen type I and IV.

Key words: spinal cord injury, Buyang Huanwu decoction, collagen type I, collagen type IV, medullary sheath, ultrastructure

CLC Number:

Lin Xiaomin1, Pan Weibin1, Wu Yuqiong1, Fan Xiao2. Effects of Buyang Huanwu decoction on the expression of collagen type I and IV after spinal cord injury [J]. Chinese Journal of Tissue Engineering Research, 2019, 23(31): 4986-4991.

Figures/Tables 5

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