Chinese Journal of Tissue Engineering Research ›› 2019, Vol. 23 ›› Issue (27): 4286-4290.doi: 10.3969/j.issn.2095-4344.1159

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Stress changes of cartilage endplate in isolated rabbit spinal motion segment under continuous pressure load

Han Tao1, 2, Zhan Jiawen1, 2, Zhu Liguo1, 2, Feng Minshan1, 2, Yin Xunlu1, 2   

  1.  (1Wangjing Hospital of China Academy of Chinese Medical Sciences, Beijing 100102, China; 2Beijing Key Laboratory of Chinese Manipulative Technique, Beijing 100700, China)
  • Received:2018-11-06 Online:2019-09-28 Published:2019-09-28
  • Contact: Zhu Liguo, Professor, Wangjing Hospital of China Academy of Chinese Medical Sciences, Beijing 100102, China; Beijing Key Laboratory of Chinese Manipulative Technique, Beijing 100700, China
  • About author:Han Tao, Master candidate, Physician, Wangjing Hospital of China Academy of Chinese Medical Sciences, Beijing 100102, China; Beijing Key Laboratory of Chinese Manipulative Technique, Beijing 100700, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81804120 (to ZJW) and 81774330 (to FMS); the National Traditional Chinese Medicine Clinical Research Base for Business Construction of State Administration of Traditional Chinese Medicine of China, No. JDZX2015274 (to ZLG); the Traditional Chinese Medicine Applied in Sports of State Administration of Sport of China, No. HXKT2017001 (to ZLG); the Beijing Traditional Chinese Medicine Orthopedic Rehabilitation Service Capacity and Technology Platform Standardization Construction Project, No. 110019 (to ZLG); the Research Project of Wangjing Hospital of China Academy of Chinese Medical Sciences, No. WJYY2018-07 (to ZJW)

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Abstract:

BACKGROUND: Changes of cartilage tissues have been shown to affect the function of intervertebral disc.
OBJECTIVE: To observe the effect of continuous pressure load on the cartilage endplate of isolated rabbit spinal segment.
METHODS: After the 16 New Zealand white rabbits were killed, the spinal movement segments were removed under the aseptic condition and randomly divided into control group (no pressure) and pressure group (continuous 29.4 N of pressure). The isolated culture was used in vitro and cultured in vitro. Before and after cultured for 3, 7 and 14 days, the 10 cartilage endplates in each group were removed for hematoxylin-eosin staining and expression of matrix metalloproteinase 13, collagen type II and proteoglycan were detected by immunohistochemistry, respectively.
RESULTS AND CONCLUSION: (1) After 14 days of culture, the cartilage structure of the intervertebral disc endplate was almost intact, the number was reduced and tended to degenerate, which showed no significant changes compared with the control group. (2) The expression of matrix metalloproteinase 13 at 14 days after culture in the pressure group was increased compared with the baseline. The average absorbance value of matrix metalloproteinase 13 in the pressure group was significantly higher than that in the control group (P < 0.05). (3) The average absorbance value of collagen type II in the pressure group was significantly higher than that in the control group at 3 days after culture (P < 0.05). The expression of collage type II after 1 and 7 days of culture in the pressure group was significantly decreased compared with the baseline, which showed no significant difference from the control group. (4) The proteoglycan staining became slight in both groups at 3, 7 and 14 days after culture (P < 0.05). The average absorbance value of proteoglycan in the pressure group was significantly higher than that in the control group at 7 days (P < 0.05). (5) In summary, continuous pressure will lead to degeneration of endplate in vitro culture, which provides evidence for the early prevention and treatment of cartilage injury in intervertebral disc degeneration.

Key words: spine, intervertebral disc, cartilage, intervertebral disc model, motor endplate, cartilage endplate, pressure load, stress changes

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