Bone defect repair with bone morphogenetic protein 14 transfected adipose-derived stem cells seeded onto a silk fibroin/hydroxyapatite scaffold

doi:10.3969/j.issn.2095-4344.0665

Abstract

Abstract:

BACKGROUND: In clinic, large bone defects are often difficult to repair naturally. Traditional methods including autologous and allogeneic bone grafts gradually show their limitations. However, the development of tissue engineering technology is expected to solve this problem. Transgenic technology is used to induce osteogenic induction of stem cells, and then induced cells are seeded onto artificial scaffold materials to construct tissue-engineered bone in vitro, which brings new hope for the repair of large-area bone defects.
OBJECTIVE: To explore the biocompatibility of bone morphogenetic protein 14 (BMP14) transfected adipose-derived stem cells with silk fibroin (SF)/hydroxyapatite (HA) scaffolds and to evaluate the effect on repairing bone defects with this tissue-engineered bone.
METHODS: Rat adipose-derived stem cells were isolated and cultured in vitro using collagenase digestion method. The third passage cells were transfected by lentivirus with BMP14 gene. Transfection efficiency was counted under fluorescent microscope. Meanwhile, the SF/HA scaffolds was prepared. Transfected cells were then seeded onto the SF/HA scaffold to prepare tissue-engineered bone. Biocompatibility of the prepared tissue-engineered bone was tested by scanning electron microscope and MTT method. Afterwards, the prepared tissue-engineered bone was implanted into the defect of the rat caudal vertebra as experimental group, and empty transfected cells with SF/HA scaffolds acted as control group. Remediation effect on bone defects was evaluated by X-ray and micro-CT examination.
RESULTS AND CONCLUSION: BMP14 transfected adipose-derived stem cells adhered to the SF/HA scaffold and grew well. At 8 weeks after surgery, X-ray and micro-CT images showed that bone density of the intervertebral space obviously increased and a large number of calli developed in the experimental group, indicating a better recovery in bone continuity, while in the control group, the scaffold material incompletely absorbed was visible in the bone defect, and no obvious new bone tissues formed to fill in the defect. To conclude, these findings reveal that the SF/HA scaffold has good biocompatibility with BMP 14-transfected adipose-derived stem cells, and the composite artificial bone has good outcomes in the repair of bone defects.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Adipose Tissue, Mesenchymal Stem Cells, Bone Morphogenetic Proteins, Silk, Hydroxyapatites, Bone Transplantation, Tissue Engineering

CLC Number:

R394.2

Ding Tao, Tang Feng-lin, Lu Miao, Gu San-jun, Wang Jian-bing, Liu Hao. Bone defect repair with bone morphogenetic protein 14 transfected adipose-derived stem cells seeded onto a silk fibroin/hydroxyapatite scaffold[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(33): 5309-5314.

Figures/Tables 2

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