Relationship between osteoporosis and incidence of knee osteoarthritis in Sprague-Dawley rats

doi:10.3969/j.issn.2095-4344.0545

Abstract

Abstract:

BACKGROUND: Incidence of osteoporosis in patients with osteoarthritis is significantly higher than that of
healthy people, indicating that there are factors leading to osteoporosis and osteoarthritis.
OBJECTIVE: To determine the effect of osteoporosis on the pathogenesis of osteoarthritis.
METHODS: Twenty-eight rats were equivalently randomized into osteoarthritis and osteoporosis plus osteoarthritis (ovariectomy to induce osteoporosis) groups. One month after modeling, papain was injected into the left knee joint of each group to induce osteoarthritis, and the right knee joint in both groups served as the controls.
RESULTS AND CONCLUSION: Compared with the right knee joint, osteophyte hyperplasia was observed at the left knee joint in both groups, and arthritis was more severe in the osteoporosis plus osteoarthritis group than in the osteoarthritis group. The Mankin scores in the osteoporosis plus osteoarthritis group were higher than those in the osteoarthritis group. The bone mineral density of L1-4 in the osteoporosis plus osteoarthritis group was lower than that in the osteoarthritis group. To conclude, osteoporosis can promote the development of osteoarthritis to some extent.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Osteoarthritis, Osteoporosis, Tissue Engineering

CLC Number:

R318

Zheng Zhao-kuan, Zhao Hong-pu, Lü Yu-ming, Zeng Jing, Gong Yi-xing, Huang Jian-wei . Relationship between osteoporosis and incidence of knee osteoarthritis in Sprague-Dawley rats[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(32): 5104-5109.

Figures/Tables 2

2.1 实验动物数量分析 28只大鼠均进入结果分析，中途无脱落。 2.2 大体观察各组大鼠右侧股骨髁关节面清楚，表面平整，有光泽，关节软骨无破坏，关节边缘无骨赘形成。骨性关节炎组：6周时大鼠的左股骨髁可见关节面清楚，表面轻度不平整，有光泽，关节软骨无破坏，关节边缘无骨赘形成。8周时大鼠的左股骨髁可见关节面毛糙模糊，表面不平整，稍有光泽，颜色稍暗红，少量局部关节软骨磨损，关节边缘有骨赘形成。骨质疏松症组+骨性关节炎组：6周时大鼠的左股骨髁可见关节面毛糙模糊，表面不平整，稍有光泽，颜色稍暗红，少量局部关节软骨磨损，关节边缘有骨赘形成。8周时大鼠的左股骨髁可见关节面毛糙模糊，表面不平整，无光泽，颜色暗红，局部关节软骨磨损，关节边缘有骨赘形成，内侧髁负重区可见软骨剥脱。 2.3 影像学结果各组大鼠左侧膝关节X射线均显示有骨赘增生、关节面不平整、关节间隙变窄等，各组右膝关节间隙对称，未见明显狭窄，骨性关节面光滑，未见明显骨赘形成(图1)。 2.4 组织病理学变化 2.4.1 苏木精-伊红染色结果 6周时，骨性关节炎组和骨质疏松症+骨性关节炎组均可见软骨表面不光滑，软骨细胞排列混乱无序，中间层和辐射层的细胞表现为轻度或中度的数量增生，潮线不完整。正常对象膝关节软骨表层的细胞扁平，呈纺锤形，在中间层的细胞圆而呈柱状，与软骨表面垂直，潮线完整。8周时，骨性关节炎组和骨质疏松症+骨性关节炎组均可见软骨表面粗糙不规则，软骨细胞数量增生明显，排列明显不规则，层次紊乱，中度细胞簇聚现象，潮线不完整。见图2。 2.4.2 番红O-固绿染色结果 6周时骨性关节炎组和骨质疏松症+骨性关节炎组均可见软骨表面不光滑，软骨细胞排列混乱无序，中间层和辐射层的细胞表现为轻度或中度的数量增生，潮线不完整。正常对照关节软骨表面平整，软骨细胞排列较规则，染色均匀。8周时，骨性关节炎组和骨质疏松症+骨性关节炎组均可见软骨表面粗糙不规则，软骨细胞数量增生明显，排列明显不规则，层次紊乱，中度细胞簇聚现象，潮线不完整(图3)。 2.5 关节软骨的Mankin评分关节软骨损伤后大鼠的Mankin评分均随时间延长而增加(P < 0.05)，且骨性关节炎+骨质疏松组大鼠Mankin评分高于骨性关节炎组(P < 0.05)。根据Mankin分级标准，骨性关节炎+骨质疏松组造模时间6周及8周时为皆为中期骨性关节炎；骨性关节炎组造模6周为早期骨性关节炎，8周为中期骨性关节炎。Mankin评分结果见表1。"

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