Proteomics analysis of osteoporosis with knee osteoarthritis

doi:10.3969/j.issn.2095-4344.0376

Abstract

Abstract:

BACKGROUND: Incidence of osteoporosis, osteoarthritis and osteoporotic osteoarthritis is high, and the relationship between osteoporosis and osteoarthritis remains controversial. Therefore, our group designed the study on the cartilages of osteoporotic osteoarthritis by proteomics based on the previous researches, so as to search for the related protein markers. It provides an objective basis for the study on osteoporotic osteoarthritis and provides theoretical basis for the prevention and treatment of osteoporosis and osteoarthritis.
OBJECTIVE: To quantitatively analyze the proteins in the knee articular cartilage tissue of New Zealand rabbit models of osteoporosis, knee osteoarthritis and osteoporotic knee osteoarthritis by proteomics iTRAQ technique, and to screen the related protein makers.
METHODS: Animal models of osteoporosis, knee osteoarthritis and osteoporotic knee osteoarthritis were established. The knee articular cartilage samples were then collected. Quantitative and qualitative analysis of proteome was conducted on these samples using proteomics iTRAQ technique. The differences of proteome among samples were compared. Specific proteins were selected which had significant differences (difference multiples > 1.2, P < 0.01) in protein expression level between comorbidity and mono-morbidity groups. In addition, characteristics of proteins as well as their relation with osteoporosis and knee osteoarthritis were analyzed by referring to uniport database and relevant literature in order to preliminarily determine the protein marker of the osteoporosis with knee osteoarthritis.
RESULTS AND CONCLUSION: A total of 12 specific proteins were screened: alpha 2-HS glycoprotein, creatine kinase M-type, fibrinogen beta chain, fibrin-ogen gamma chain, serum albumin, vitamin D binding protein, N-acylethanol-amine acid amidase, tubulin polymerization-promoting protein family member 2, asporin protein, myelin protein zero, uncharacterized protein (G1TQR3) and uncharacterized protein (U3KLT3). The above 12 proteins were significantly increased in the osteoporotic knee osteoarthritis group. These results indicate that eight specific proteins were identified, and their biological features are closely related to the pathogenesis of osteoporosis and knee osteoarthritis. The protein markers of osteoporosis with knee osteoarthritis were identified preliminarily, which can be used for the material basis for further study.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程
ORCID: 0000-0002-1545-2304(时刚)

Key words: Osteoporosis, Osteoarthritis, Tissue Engineering

CLC Number:

R318

Shi Gang1, Zhang Kaiwei2. Proteomics analysis of osteoporosis with knee osteoarthritis[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(36): 5753-5759.

Figures/Tables 5

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