Abstract:

BACKGROUND: A key problem of study on tracheal prosthesis is to cover the tracheal mucosa and recover its function. Stromal cell derived factor-1/CXCR4 pathway plays an important role in accelerating tissue repair.
OBJECTIVE: To investigate the effects of stromal cell derived factor-1 on the proliferation and directional migration of bronchial epithelial cells transfected by CXCR4 gene.
METHODS: CXCR4 lentiviral vector was constructed and transfected into human bronchial epithelial cells. In the blank group, cells were not infected with any virus. In the control group, cells were infected with non-transfected lentiviral vectors. In the experimental group, cells were infected with CXCR4 lentiviral vectors. CXCR4 lentiviral vectors-transfected and common human bronchial epithelial cells were processed with different concentrations of stromal cell derived factor-1 (1 μmol/L, 100, 10, 1, 0.1, 0 nmol/L).
RESULTS AND CONCLUSION: CXCR4 lentiviral vector was successfully constructed. CXCR4 protein expression was low in the normal human bronchial epithelial cells. CXCR4 mRNA and protein expression was high in the CXCR4 gene- transfected human bronchial epithelial cells. With increasing concentration of stromal cell derived factor-1, absorbance was gradually increased in a concentration-dependent manner (P < 0.05). This suggests that stromal cell derived factor-1 promotes the proliferation and directional migration of bronchial epithelial cells transfected by CXCR4 gene.