Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (24): 4447-4452.doi: 10.3969/j.issn.1673-8225.2012.24.016

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Demonstration empirical study of the corticospinal tracts by biotinylated dextran amine tracer

Ba Ying-chun1, Tang Jian-zhong2, Fan Yan1, Li Zhong-ming1, Wang Jin-de1, Wang Ting-hua3   

  1. 1Department of Human Anatomy, 2Institute of Neuroscience, Kunming Medical College, Kunming 650500, Yunnan Province, China;
    3Department of Hepatology, First People’s Hospital of Yunnan Province, Kunming 650032, Yunnan Province, China
  • Received:2012-04-13 Revised:2012-04-30 Online:2012-06-10 Published:2013-11-05
  • Contact: Wang Ting-hua, Doctor, Professor, Department of Hepatology, First People’s Hospital of Yunnan Province, Kunming 650032, Yunnan Province, China tinghua_neuron@263.net
  • About author:Ba Ying-chun☆, Doctor, Associate professor, Department of Human Anatomy, Kunming Medical College, Kunming 650500, Yunnan Province, China byc71206@yahoo.com.cn

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Abstract:

BACKGROUND: Biotinylated dextran amine mechanisms and metabolic processes are not entirely clear, which may be similar to the complex linked with biotin.
OBJECTIVE: To choose the best biotinylated dextran amine tracing empirical method to fully show courser and distribution of the corticospinal tracts
METHODS: Fifteen normal Sprague-Dawley female rats were used in this study and divided into three groups, with five rats in each group: in the first group, rats survived for 2 weeks after 10% biotinylated dextran amine (0.5 μL/site) was injected into the bilateral cerebral cortices; while 10% biotinylated dextran amine (1 μL/site) was injected into the right cerebral cortex in the second group rats which survived for 3 weeks; in the third group, 10% biotinylated dextran amine (0.5 μL/site) was injected into the bilateral cerebral cortices, and the rats survived for 3 weeks.
RESULTS AND CONCLUSION: The trace effect of biotinylated dextran amine in the third group was the best. This indicates that the tracing effect of biotinylated dextran amine is optimal in the rats receiving multi-point injection of biotinylated dextran amine (10%, 0.5 μL/site) into the bilateral cerebral cortices and surviving for 3 weeks after injection.

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