Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (24): 4441-4446.doi: 10.3969/j.issn.1673-8225.2012.24.015

Previous Articles     Next Articles

Role of nuclear factor kappa B signaling pathway in stretch-induced differentiation of C2C12 myoblasts

Yan Xiao1, Li Fei-fei2, Liu Li-juan1, Sun Xian-rui1, Zhang Yue1, Liu Wen1, Yao Ru-yong3, Yuan Xiao1   

  1. 1Department of Stomatology, the Affiliated Qingdao Municipal Hospital, Medical College of Qingdao University, Qingdao 2660275, Shandong Province, China; 2Stomatology College of Shandong University, Jinan 250000, Shandong Province, China;
    3Central Laboratory, the Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China
  • Received:2011-12-02 Revised:2012-02-02 Online:2012-06-10 Published:2013-11-05
  • Contact: Yuan Xiao, Doctor, Associate professor, Master’s supervisor, Department of Stomatology, the Affiliated Qingdao Municipal Hospital, Medical College of Qingdao University, Qingdao 2660275, Shandong Province, China yuanxiaoqd@163.com
  • About author:Yan Xiao★, Studying for master’s degree, Department of Stomatology, the Affiliated Qingdao Municipal Hospital, Medical College of Qingdao University, Qingdao 2660275, Shandong Province, China yuanxiao0409@163.com

PDF

614

Abstract:

BACKGROUND: Nuclear factor kappa B (NF-κB) is a convergence point of several signaling pathway, playing an important role in cell growth and differentiation, inflammatory response, and tumor growth, as well as regulation of myoblasts differentiation.
OBJECTIVE: To investigate the effect and action mechanism of NF-κB signaling pathway on stretch-induced differentiation of C2C12 myoblasts.
METHODS: In vitro culture-tensile stimulate models of C2C12 myoblasts were established successfully by using a multi-passage load adding system. Cyclic stretch was applied on the myoblasts for 2, 6, 12 and 24 hours at an optimal magnitude (10%) and frequency (0.5 Hz).
RESULTS AND CONCLUSION: ①NF-κB signaling pathway was activated when C2C12 myoblasts were induced by cyclic stretch. The expression of p65 subunit of NF-κB was increased after 6 hours of mechanical stretch, reaching a maximum after 24 hours of mechanical stretch, which was significantly different from the control groups (P < 0.05), confirming a correlation between NF-κB activation and stretch-induced muscle differentiation. ②To determine whether IκBα degradation occurred in response to mechanical stretch, C2C12 cells were exposed to cyclic stretch for various time points and total protein extracts were analyzed by Western blot analysis. A significant reduction in the levels of IκBα was observed after 6 hours of mechanical stretch, reaching a minimum after 24 hours of mechanical stretch, significantly different from the control groups (P < 0.05). ③Treatment of cells with the specific NF-κB inhibitor, pyrrolidine dithiocarbamate, could decrease the expression of myogenin, but not completely abolish the myogenin expression after stretch. These results indicated that: ①NF-κB may be involved in stretch-induced C2C12 cell differentiation. ②Phosphorylation and subsequent degradation of IκBα occurs on stimulation of the cells. ③NF-κB signaling pathway plays an important role during stretch-induced C2C12 cell differentiation, but is not activated exclusively in this process.

CLC Number: