Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (24): 4437-4440.doi: 10.3969/j.issn.1673-8225.2012.24.014

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Antibacterial effect of purified maggot secretion antimicrobial peptides on ulcer wound of diabetic rats

Gao Lei1, Yin Ye-feng1, Wang Shou-yu2, Wang Jiang-ning1, 3   

  1. 1Dalian University, Dalian 116622, Liaoning Province, China;
    2Department of Orthopedics, the First Affiliated Hospital of Dalian Medical University, Dalian 116011, Liaoning Province, China;
    3Beijing Luhe Hospital of Capital Medical University, Beijing 101100, China
  • Received:2011-11-10 Revised:2011-12-20 Online:2012-06-10 Published:2013-11-05
  • Contact: Wang Jiang-ning, Doctor, Chief physician, Dalian University, Dalian 116622, Liaoning Province, China; Beijing Luhe Hospital of Capital Medical University, Beijing 101100 dlwangjn@vip. sina.com
  • About author:Gao Lei★, Studying for master’s degree, Dalian University, Dalian 116622, Liaoning Province, China tjyd1026@163.com

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Abstract:

BACKGROUND: Maggot secretions have a good antibacterial effect on infected wounds. The studies have confirmed that there are antimicrobial peptides that can inhibit and kill the bacteria in the maggot secretions, and we have carried out effective purification of the peptide.
OBJECTIVE: To investigate the antibacterial effect and wound healing of purified maggot secretion antimicrobial peptides on ulcer wound of diabetic rats.
METHODS: Twenty 3.5-month-old male SD rats weighing 330-370 g was used to prepare diabetic ulcer wound model. The rats were randomly divided into two groups (n=10). In the experimental group, the wounds were coated with purified maggot secretion antimicrobial peptide; the control group was not treated.
RESULTS AND CONCLUSION: In the experimental group, the wounds were clean and healed well with fresh granulation, no purulent secretions and no staphylococcus aureus infections. In the control group, wound exudates were found with severe erosion, and the wound was enlarged and deepened that healed poor. The staphylococcus aureus infection rate in the control group was 70%. After 7, 14, 21 and 28 days, the ulcer area in the experimental group was significantly smaller than that in the control group (P < 0.05). It indicates that the purified maggot secretion antimicrobial peptide can promote the ulcers wound healing of diabetic rats and prevent the tissue bacterial infection.

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