Abstract:

BACKGROUND: The occurrence and development of hypertrophic scar may be related with the abnormal expression of cell cycle related genes.
OBJECTIVE: To detect the mRNA and protein expression of CyclinD1, CDK2 and CDK4 in fibroblasts at different stages of hypertrophic scar; to explore the consistency of CyclinD1, CDK2 and CDK4 in fibroblasts of the same hypertrophic scar during the cell cycle.
METHODS: A total of 32 hypertrophic scar samples were collected and divided into four groups based on their formation stages of hypertrophic scar: 3 months group, 6 months group, 1 year group and 2 years group, 8 samples for each group; eight normal skin samples were used as control. The mRNA and protein expression of CyclinD1, CDK2 and CDK4 were detected using quantitative real-time PCR and wetern blot. The cell cycle of fibroblasts was detected using flow cytometer.
RESULTS AND CONCLUSION: With the development of hypertrophic scar, the mRNA and protein expression level of CyclinD1, CDK2 and CDK4 in hypertrophic scars changed from strong to weak. Most fibroblasts in hypertrophic scars of the 3 months group and 6 months group were found in the S phase and G2/M phase; most fibroblasts in hypertrophic scars of the 1 year group and 2 years group were found in the G0/G1 phase. These findings indicate that the mRNA and protein expression tendencies of CyclinD1, CDK2 and CDK4 are basically consistent at different stages of hypertrophic scar; meanwhile, the distribution of cell cycle in fibroblasts of hypertrophic scars corresponds with the functions of CyclinD1, CDK2 and CDK4.