Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (24): 4376-4380.doi: 10.3969/j.issn.1673-8225.2012.24.002

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Bone morphogenetic protein-4 gene polymorphism associated with ossification of the thoracic ligamentum flavum

Zhao Wei-guang1, Liu Zhen-wu1, Liu Li1, Xie Yan-ping1, Jiang Li-qiang1, Lin Xin2   

  1. 1Department of Orthopedics, Handan Central Hospital, Handan 056001, Hebei Province, China;
    2Department of Orthopedics, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China
  • Received:2012-04-01 Revised:2012-04-17 Online:2012-06-10 Published:2013-11-05
  • Contact: Lin Xin, Doctoral supervisor, Chief physician, Department of Orthopedics, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China linxin@bjttyy.com
  • About author:Zhao Wei-guang★, Master, Physician, Department of Orthopedics, Handan Central Hospital, Handan 056001, Hebei Province, China zhaoweiguang361@163.com

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Abstract:

BACKGROUND: There are few studies addressing the association of bone morphogenetic protein-4 gene and ossification of the thoracic ligamentum flavum (OLF).
OBJECTIVE: To explore whether two single nucleotide polymorphisms of the bone morphogenetic protein-4 were related to the morbidity and severity of OLF.
METHODS: The venous blood samples of 40 OLF patients and 40 non-OLF controls were collected. All experimental participants were matched in age-sex composition. The DNA extracted from the peripheral venous blood was amplified and sequenced at two sites of rs17563 and rs2855532 by using PCR.
RESULTS AND CONCLUSION: The frequency of “T” genotype and allelotype at rs17563 and rs2855532 in the OLF patients was higher than that in the non-OLF patients (P < 0.05). The two single nucleotide polymorphisms (rs17563 and rs2855532) can increase the incidence of OLF.

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