Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (24): 4371-4375.doi: 10.3969/j.issn.1673-8225.2012.24.001

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Histopathological changes of SD rat skeletal muscle in the early stage of contusion

Liu Shu-kun1, Yu Xiao-hua2, Wu Yao-yi3, Xu Ming-ming1, Zhao Zhu-ying2, Luo Xi2, Ma Chuan-yu1   

  1. 1Clinical Medical College of Jiangsu University, Zhenjiang 212001, Jiangsu Province, China;
    2Military Training Medical Institute of Nanjing Military Region, the 101 Hospital of Chinese PLA, Wuxi 214044, Jiangsu Province, China;
    3Department of Pathology, the 101 Hospital of Chinese PLA, Wuxi 214044, Jiangsu Province, China
  • Received:2011-11-07 Revised:2011-11-24 Online:2012-06-10 Published:2013-11-05
  • Contact: Yu Xiao-hua, Professor, Chief physician, Master’s supervisor, Military Training Medical Institute of Nanjing Military Region, the 101 Hospital of Chinese PLA, Wuxi 214044, Jiangsu Province, China yuxiaohua-101@163.com
  • About author:Liu Shu-kun★, Studying for master’s degree, Clinical Medical College of Jiangsu University, Zhenjiang 212001, Jiangsu Province, China lsk541982@126.com

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Abstract:

BACKGROUND: Effects of keeping exercise after skeletal muscle contusion on regeneration of muscular tissue remain unclear.
OBJECTIVE: To investigate the effects of treadmill running on the histopathology of skeletal muscle in SD rats with acute contusion.
METHODS: A total of 64 male SD rats were randomly divided into four groups: normal control group, model group, natural healing group and exercise intervention group. Skeletal muscle contusion model in the middle part of the hindlimb gastrocnemius was constructed in the latter three groups. Rats in the exercise intervention group underwent treadmill exercise; rats in the natural healing group received normal feeding without exercise intervention. Rats in the model group were sacrificed at the 6th hour after model construction.
RESULTS AND CONCLUSION: Massive inflammation cells were found in rat damaged muscle tissues of the natural healing group and exercise intervention group in the 24th hour to the 3rd day after acute gastrocnemius contusion injury; the inflammation decreased on the 5th day. The number of inflammation cells in the exercise intervention group was significantly larger than that in the natural healing group at different time points of the same period (P < 0.05). The inflammation cells disappeared on the 7th day after injury in both groups. A small amount of scar tissue was found on the 5th day after injury in both groups, which increased gradually with time. The area of fibrous scar in the exercise intervention group was significantly larger than that in the natural healing group on the 7th to 14th days after injury (P < 0.01); there was still a growing trend in the scar tissues of both groups on the 14th day after injury. It indicate that exercise can aggravate the local inflammation and lead to excessive formation of scar tissue in the early regeneration following acute contusion of skeletal muscle; it has a negative impact on the repair of skeletal muscle.

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