Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (21): 3864-3868.doi: 10.3969/j.issn.1673-8225.2012.21.015

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Feasibility of acellular dermal matrix as a dermal substitute in tissue engineering 

Liu Po1, Qi Shao-hai2, Shu Bin2, Xie Ju-lin2, Xu Ying-bin2, Liu Xu-sheng2   

  1. 1The First Department of Surgery, Tangxia Hospital, Dongguan  523721, Guangdong Province, China; 2Department of Burns, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou  510080, Guangdong Province, China
  • Received:2011-11-01 Revised:2012-01-07 Online:2012-05-20 Published:2012-05-20
  • Contact: Qi Shao-hai, Master, Professor, Doctoral supervisor, Department of Burns, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China qishaohai@yahoo.com.cn
  • About author:Liu Po★, Master, the First Department of Surgery, Tangxia Hospital, Dongguan 523721, Guangdong Province, China zsylper@yahoo.com.cn
  • Supported by:

     the Scientific and Technological Foundation of Guandong Province, No. 2010B031100008*

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Abstract:

BACKGROUND: Tissue engineered skin has been used to repair defect skin. Acellular dermal matrix (ADM) had no immunogenicity, which cannot lead to rejection following transplantation, thus, this is an ideal dermal substitute for tissue engineered skin.
OBJECTIVE: To explore the biocompatibility of ADM.　
METHODS: The histocompatibility, hydrophilicity and mechanical performance of ADM were detected by cytotoxicity test in vitro and vivo, equilibrium water content, swelling degree and biomechanical analysis.　
RESULTS AND CONCLUSION: No cell component was observed in the ADM. The ventage of ADM was 100-180 µm in diameter. In the experimental group, equilibrium water content was (69.6±3.97)%, swelling degree was 2.3±0.42 and the breakage force was (3.082±0.046) N. There was no significant difference between experimental and control groups (P > 0.05). It was indicated that in cytotoxicity test, no inhibition effect on dermal papilla cells in vitro and no severe immunological rejection in vivo was found. The selected ADM could be an optimal biological material as a substitute of dermal with good histocompatibility, low immunogenicity and moderate mechanical performance.

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