Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (21): 3855-3858.doi: 10.3969/j.issn.1673-8225.2012.21.013

Previous Articles     Next Articles

Construction of tissue engineering bladder mucosa by collagen sponge in vitro

Zhang Ming1, Xu Ming-xi1, Wu Jia-sheng2, Li Wei2, Sun Kang2, Wang Zhong1, Zhou Zhe1, Lu Mu-jun1   

  1. 1Department of Urology, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai  200011, China; 2State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai  200240, China
  • Received:2011-10-19 Revised:2011-12-20 Online:2012-05-20 Published:2012-05-20
  • Contact: Lu Mu-jun, Associate chief physician, Master’s supervisor, Department of Urology, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China lumujun@163.com Sun Kang, Professor, Doctoral supervisor, State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China ksun@sjtu.edu.cn
  • About author:Zhang Ming★, Studying for master’s degree, Department of Urology, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China zhangm4911@ 163.com Xu Ming-xi★, Studying for master’s degree, Attending physician, Department of Urology, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China xmx197630@ 163.com
  • Supported by:

    the National Natural Science Foundation of China, No.81070605*; Biomedical Engineering Research Fund of Shanghai Jiao Tong University, No. YG2011MS14*; Research and Innovation Projects of Shanghai Municipal Education Commission, No.09YZ76*

PDF

410

Abstract:

BACKGROUND: Construction of tissue engineering bladder mucosa is very important in the repair of tissue engineering bladder. But there is no reasonable repair method up to now.
OBJECTIVE: To construct the bladder mucosa by collagen sponge scaffold combined with porcine bladder cells.
METHODS: The urothelial cells were scraped from the porcine urinary bladder mucosa, and digested by trypsin for primary culture. The marker of urothelial cells at passage three were detected by immunofluorescence and reverse transcription-PCR. Porous collagen sponge scaffolds were made. The urothelial cells at passage three were seeded on the collagen sponge scaffolds. After cultured for 4-8 days, the cell-collagen sponge composites were observed.
RESULTS AND CONCLUSION: Primary cultured porcine bladder urothelial cells were polygon and paving stone-like, and they could form the clone groups. Immunofluorescence identification of AE1/AE3 was positive, reverse transcription-RCR detection of uroplakin-IA and uroplakin-Ⅱ were positive. The histological study found that after cultured for 4-8 days, the cells grew well on collagen sponge scaffold, the cells covering the surface of collagen materials and growing into the scaffold maintained the characteristics of urothelial cells. After cultured for 6 days in vitro, the cell-seeded collagen scaffold grew best with the highest number of cells compared to 4 and 8 days. Urothelial cell-seeded collagen sponge is a suitable material for the repair of bladder mucosa by tissue engineering technology in vitro. The best time of culture before replantation in vitro is 6 days.

CLC Number: