Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (19): 3433-3437.doi: 10.3969/j.issn.1673-8225.2012.19.003

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Estrogen promotes osteogenic differentiation of mouse bone marrow mesenchymal stem cells in a dose-dependent manner  

Zhu Xiao-fei1, Wang Yang2, Jin Yan3, Jia Li-hui4   

  1. 1Postgraduate Culture Base, the General Hospital of Shenyang Military Region, Liaoning Medical University, Shenyang  110016, Liaoning Province, China; 2Department of Plastic Surgery, 4Department of Training, the General Hospital of Shenyang Military Region, Shenyang  110016, Liaoning Province, China; 3Tissue Engineering Center, School of Stomatology, Fourth Military Medical University of Chinese PLA, Xi’an   710032, Shaanxi Province, China
  • Received:2012-03-01 Revised:2012-03-19 Online:2012-05-06 Published:2012-05-06
  • Contact: Jia Li-hui, Master’s supervisor, Associate chief physician, Department of Training, the General Hospital of Shenyang Military Region, Shenyang 110016, Liaoning Province, China
  • About author:Zhu Xiao-fei★, Studying for master’s degree, Postgraduate Culture Base, the General Hospital of Shenyang Military Region, Liaoning Medical University, Shenyang 110016, Liaoning Province, China

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Abstract:

BACKGROUND: The pathogenesis of postmenopausal osteoporosis and estrogen deficiency is closely related.
OBJECTIVE: To investigate the effects of different concentrations of estrogen on osteogenic differentiation of mouse bone marrow mesenchymal stem cells (BMSCs) and the relationship with miRNA-26a.
METHODS: Mouse femoral and tibial bone marrow was taken and BMSCs were isolated and purified by the whole bone marrow adherence method. Estrogen at different concentrations (0, 10-10, 10-9, 10-8, 10-7, 10-6 mol/L) was used for osteogenic induction.
RESULTS AND CONCLUSION: Estrogen did not greatly influence the proliferation of BMSCs, but it could significantly enhance the osteogenic differentiation capability of BMSCs. At the same time, estrogen, in particular at a concentration of 10-9 mol/L, could promote RUNX2, OCN mRNA and RUNCX2, SP7 protein expression in BMSCs. However, 10-9 mol/L estrogen promoted miRNA-26a mRNA expression at the weakest capability. These findings suggest that estrogen can promote osteogenic differentiation of BMSCs in a dose-dependent manner, and microRNA-26a possibly exerts effects during this process.

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