Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (19): 3427-3432.doi: 10.3969/j.issn.1673-8225.2012.19.002

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Bone marrow mesenchymal stem cells increased expression of urokinase type plasminogen activator and promoted apoptosis of hepatic stellate cells in rats   

Ning Lin, Jiang Hai-xing, Qin Shan-yu, Zhang Jun-hong, Yang Wen, Meng Yun-chao   

  1. Department of Digestive Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2012-02-09 Revised:2012-03-09 Online:2012-05-06 Published:2012-05-06
  • Contact: Jiang Hai-xing, M.D., Professor, Doctoral supervisor, Department of Digestive Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China jihaxi@163.com
  • About author:Ning Lin★, Studying for master’s degree, Department of Digestive Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China 1223163675@qq.com
  • Supported by:

    the Natural Science Foundation of Guangxi Zhuang Autonomous Region, No. 0897008*; the New Centuary National Hundred, Thousand and Ten Thousand Talent Project, No. 2006206*

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Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) promote the apoptosis of hepatic stellate cells (HSCs) by paracrine and activation of hepatocyte growth factor (HGF). 
OBJECTIVE: To investigate the effects of BMSC regulation to urokinase type plasminogen activator on activity of hepatocyte growth factor, and the mechanism by which BMSCs induce the apoptosis of hepatic stellate cells.
METHODS: Five groups were divided randomly: Co-culture group, in which rat BMSCs and HSCs were co-cultured by upper and down double-layer co-culture system; HSCs group, in which HSCs were simply cultured; fibroblasts group; UK122 pretreated group: 6 hours before co-culture of BMSCs and HSCs, urokinase type plasminogen activator specific inhibitor UK122 was added; BMSCs blank control group.
RESULTS AND CONCLUSION: mRNA expression of urokinase type plasminogen activator in the co-culture group was significantly higher than in the HSCs group (P < 0.01). Compared with HSC group, HSC proliferation was significantly inhibited in the co-culture group at 24 hours (P < 0.01) in a time-dependent manner. After co-culture, HGF protein expression and HSC apoptosis were significantly increased (P < 0.01). After UK122 intervention, HGF expression and HSC apoptosis were significantly decreased (P < 0.01). These findings suggest that BMSCs promote the secretion of urokinase type plasminogen activator, increase the expression of active HGF, and promote the apoptosis of HSCs.

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