Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (18): 3323-3328.doi: 10.3969/j.issn.1673-8225.2012.18.021

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Effect of heat shock protein 27, erythropoietin and heme oxygenase-1 activated by remote ischemic preconditioning on kidney ischemia/reperfusion injury  

Li Yan1, Wu Jia-qing1, Shen Sheng1, Meng Shan-dong1, Zhou Jie-xue1, Zhang Ming2, Liu Dong1   

  1. 1Organ Transplant Center, Affiliated Guangdong Provincial N0.2 People’s Hospital, Medical School of University of South China, Guangzhou  510317, Guangdong Province, China; 2Deparment of Liver Surgery, Affiliated Renji Hospital, Medical College of Shanghai Jiao Tong University, Shanghai  200127, China
  • Received:2011-12-11 Revised:2012-02-09 Online:2012-04-29 Published:2012-04-29
  • Contact: Liu Dong, Chief physician, Organ Transplant Center, Affiliated Guangdong Provincial N0.2 People’s Hospital, Medical School of University of South China, Guangzhou 510317, Guangdong Province, China antslydf@163.com
  • About author:Li Yan★, Master, Organ Transplant Center, Affiliated Guangdong Provincial N0.2 People’s Hospital, Medical School of University of South China, Guangzhou 510317, Guangdong Province, China pioneer00.lee00@gmail.com
  • Supported by:

    The First Batch of Industrial Technology Research and Development Funds of Guangdong Province in 2010, No.2010B08070 1048*; Natural Science Foundation of Guangdong Province in 2011, No.S201101000 1333*

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Abstract:

BACKGROUND: Ischemia-reperfusion injury is the important reasons for acute renal failure and other disease, and the mechanism is mainly the multi-factor and multi-channel complex pathological process.
OBJECTIVE: To observe the influence of the heat shock protein 27 (HSP27), erythropoietin and heme oxygenase 1 activated by remote ischemic preconditioning in kidney ischemia/reperfusion injury.
METHODS: Ninety male C57BL/6 mice were randomly divided into three groups: ischemia/reperfusion group (n=30), preconditioning group (n=30), sham operation group (n=30). Mice in the ischemia/reperfusion group were removed the right kidney and the left kidney were subjected to ischemia for 25 minutes, and reperfusion for 24 hours; mice in the preconditioning group were subjected to bilateral renal ischemia for 20 minutes and were exposed to ischemia/reperfusion surgery on day 8 after reperfusion. Abdominal aortic blood was taken to test kidney function.
RESULTS AND CONCLUSION: Serum creatinine and blood urea nitrogen of preconditioning group and the sham operation group were significantly lower than that of the ischemia reperfusion group (P < 0.01); MPO staining found a large number of neutrophils infiltration in ischemia/reperfusion group (P < 0.01); PAS staining showed that the kidney histopathology of preconditioning group was better than that of ischemia reperfusion group (P < 0.05); TUNEL staining analysis displayed that the apoptosis cells in preconditioning and sham operation group were significantly less than that in the ischemia/reperfusion group   (P < 0.01); HSP27 mRNA expression in preconditioning group was significantly higher than that in the ischemia/reperfusion and sham operation group (P < 0.01), the peak of HSP27 mRNA expression was at 8 days, erythropoietin and heme oxygenase 1 mRNA were increased to A point during 24 and 48 hours, and then gradually decreased, but it was taken to another peak point B at 8 days and B was more higher than A, and the peak B in the preconditioning group was higher than that in the sham operation group (P < 0.01). Remote ischemic preconditioning activated HSP27, erythropoietin and heme oxygenase 1 mRNA expression can take part in reducing infiltration of inflammatory cytokines, promoting the repair of tubular cells and inhibiting the tubular cell apoptosis in order to protected the kidney ischemia/reperfusion injury.
 

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