Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (16): 2898-2900.doi: 10.3969/j.issn.1673-8225.2012.16.011

Previous Articles     Next Articles

Drug content and entrapment efficiency of elemene microemulsion detected by ultra-fast liquid chromatography and liquid surface method

Liu Xing-yan1, Liu Hong1, Zhou Guang-lin2, Zhan Xiao-ri2, Liu Jun2, Zeng Zhao-wu1, Ding Yuan-lin1   

  1. 1Guangdong Medical College, Dongguan  523808, Guangdong Province, China; 2Hangzhou Normal University, Hangzhou  310012, Zhejiang Province, China
  • Received:2012-01-04 Revised:2012-02-24 Online:2012-04-15 Published:2012-04-15
  • Contact: author: Ding Yuan-lin, Doctor, Professor, Guangdong Medical College, Dongguan 523808, Guangdong Province, China artgreenking@ 126.com
  • About author:Liu Xing-yan☆, Doctor, Associate researcher, Guangdong Medical College, Dongguan 523808, Guangdong Province, China lxy0428@126.com
  • Supported by:

    the National Youth Science Foundation of China, No. 81001647*; the Natural Science Foundation of Guangdong Province, No. 9151051501000035*; the Foundation of the Science and Technology Bureau of Zhejiang Province, No. 2009C33005*; the General Program of China Postdoctoral Science Foundation, No. 20100471757*

PDF

289

Abstract:

BACKGROUND: Ultra-fast liquid chromatography is fast and accurate to detect content of elemene microemulsion.
OBJECTIVE: To detect the drug content and entrapment efficiency of elemene microemulsion using ultra-fast liquid chromatography and liquid surface method.
METHODS: The drug content of elemene microemulsion was determined by ultra-fast liquid chromatography. As elemene was insoluble in water and floated on water, liquid surface method was designed to detect the encapsulation efficiency of elemene emulsion.
RESULTS AND CONCLUSION: It was better for β-elemene to separate from accessories under the chromatographic conditions, and β-elemene at a dose of 3.94-27.58 mg/L had a good linear relationship. The elemene microemulsion was characterized as (8.273 ± 0.018) g/L in content of β-elemene, and (99.81±0.24)% in average entrapment efficiency. The present study demonstrates that ultra-fast liquid chromatography and liquid surface method are simple, fast and accurate for determining the drug content and entrapment efficiency of elemene microemulsion. The elemene microemulsion has high entrapment efficiency.

CLC Number: