Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (15): 2745-2749.doi: 10.3969/j.issn.1673-8225.2012.15.019

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Effect of low-energy extracorporeal shock wave on the expression of vascular endothelial growth factor and angiogenesis in diabetic wound healing  

Yan Xiao-yu, Yang Guang, Cheng Liang, Chen Ming, Cheng Xiang-guo, Chai Yi-min, Luo Cong-feng, Zeng Bing-fang   

  1. Department of Orthopedics, the Sixth Affiliated People’s Hospital of Shanghai Jiao Tong University, Shanghai  200233, China
  • Received:2011-12-24 Revised:2012-01-13 Online:2012-04-08 Published:2012-04-08
  • Contact: author: Chai Yi-min, Doctor, Chief physician, Department of Orthopedics, the Sixth People’s Hospital of Shanghai Jiao Tong University, Shanghai 200233, China chaiyimin2000@yahoo.com.cn
  • About author:Yan Xiao-yu☆, Doctor, Attending physician, Department of Orthopedics, the Sixth Affiliated People’s Hospital of Shanghai Jiao Tong University, Shanghai 200233, China yanorth@126.com
  • Supported by:

    National Natural Science Foundation of China, No. 30901565*; Youth Science and Technology of Shanghai Rising Star Program (A class) Project, No. 10QA1405400*

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Abstract:

BACKGROUND: Extracorporeal shock wave (ESW) as a non-invasive physical stimulation can promote angiogenesis and tissue repair.
OBJECTIVE: To observe the effect of low-energy ESW on vascular endothelial growth factor (VEGF) expression and its role in promoting diabetic wound healing.
METHODS: Totally 72 Sprague Dawley rats were randomly divided into treatment, diabetes and control groups. Diabetic chronic wound models were established in the treatment and diabetes groups. The ESW was used for the treatment group at 1 day after modeling, but coupling liquid was used for the diabetes and control groups. Granulation tissue of the wound and formation of new blood vessels were observed. Protein content of VEGF and expression of mRNA were detected.
RESULTS AND CONCLUSION: Compared with the diabetes group, closure rate of the wound in the treatment group was increased. The number of microvessels within the wound was increased in the treatment group at 3 days after treatment than in the diabetes group, and corresponding granulation tissues were increased simultaneously. Compared with the control group, expressions of VEGF protein and mRNA were lower in the diabetic control group at 3 and 7 days after treatment, moreover, declined at 7 days after treatment. After the ESW treatment, the protein content of VEGF and expression of mRNA level were increased at 3, 7, 14 and 21 days after treatment and began to decline at 14 days after treatment. This suggested that the reduced expression level of VEGF and decreased period of its expression can contribute to the impaired healing of diabetic wound. The ESW treatment can increase the expression of VEGF and extend the time of its high expression to promote wound angiogenesis and ultimately accelerate the growth of granulation tissue and diabetic wound healing.
Yan XY, Yang G, Cheng L, Chen M, Cheng XG, Chai YM, Luo CF, Zeng BF. Effect of low-energy extracorporeal shock wave on the expression of vascular endothelial growth factor and angiogenesis in diabetic wound healing. Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(15): 2745-2749.     [http://www.crter.cn  http://en.zglckf.com]
 

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