Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (15): 2741-2744.doi: 10.3969/j.issn.1673-8225.2012.15.018

Previous Articles     Next Articles

Effect of hepatocyte growth factor on the proliferation and apoptosis of primary hepatic stellate cells under tumor necrosis factor-related apoptosis-inducing ligand

Zhang Jun-hong, Jiang Hai-xing, Qin Shan-yu, Lu Zheng-feng, Meng Yun-chao, Ning Lin, Yang Wen   

  1. Department of Gastroenterology, the First Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2011-09-16 Revised:2011-10-29 Online:2012-04-08 Published:2012-04-08
  • Contact: author: Jiang Hai-xing, Professor, Doctoral supervisor, Department of Gastroenterology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China jihaxi@163.com
  • About author:Zhang Jun-hong☆, Studying for doctorate, Attending physician, Department of Gastroenterology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China junjun12162001@163.com
  • Supported by:

    the Natural Science Foundation of Guangxi, No. 0897008*; Self-funded Research Projects, Health Department of Guangxi Zhuang Autonomous Region, No. Z2011317*  

PDF

291

Abstract:

BACKGROUND: Activated hepatic stellate cells play a key role in liver fibrosis. Research shows that hepatocyte growth factor (HGF) can promote the apoptosis of activated hepatic stellate cells and the specific mechanisms may have relationship with the apoptosis of enhanced-related apoptosis-inducing ligand (TRAIL)-induced stellate cells.
OBJECTIVE: To investigate the role of HGF under the action of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) in primary hepatic stellate cells (HSCs) proliferation, apoptosis and to explore the possible mechanisms involved.
METHODS: Primary HSCs of SD rats were used to recovery and passage, and were used in the experiment when the proliferation was obvious. HSCs were divided into four groups: ① blank control group, HSCs cultured alone; ② HGF group,    100 μg/L HGF was injected into HSCs; ③ TRAIL group, 2 mg/L TRAIL was injected into HSCs; ④ HGF+TRAIL group, the HSCs were prestimulated by HGF for 24 hours and then 2 mg/L TRAIL was injected into HSCs.
RESULTS AND CONCLUSION: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed that HGF at 50- 200 μg/L and TRAIL at 0.5-1.5 mg/L had no effect on HSCs proliferation. 2 mg/L rate TRAIL could inhibit HSCs proliferation. Mid and late apoptosis of HSCs was detected by flow cytometry, the apoptosis rate in the HGF+TRAIL group was higher than that in the blank control group and the HGF group (P < 0.05); the DR5 fluorescence intensity in the HGF+TRAIL group was higher than that in the blank control group, the HGF group and the TRAIL group (P < 0.01). Under the action of the TRAIL, HGF could promote the apoptosis of HSCs and inhibit the proliferation. The possible machanism was that HGF could increase the expression of DR5 on HSCs surface.
Zhang JH, Jiang HX, Qin SY, Lu ZF, Meng YC, Ning L, Yang W. Effect of hepatocyte growth factor on the proliferation and apoptosis of primary hepatic stellate cells under tumor necrosis factor-related apoptosis-inducing ligand. Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(15): 2741-2744.     [http://www.crter.cn  http://en.zglckf.com]

CLC Number: