Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (15): 2715-2719.doi: 10.3969/j.issn.1673-8225.2012.15.012

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Effect of ozone on hypertrophic scar during the healing of bilateral circular full-thickness skin wounds on the back of rats

Ou Shan-xing1, Li Xiao-rong1, Zhang Li1, Yu Zhi-jian2   

  1. 1Department of Radiology, Guangzhou General Hospital of Guangzhou Military Command of Chinese PLA, Guangzhou  510282, Guangdong Province, China; 2Department of Image, Zhujiang Hospital, Southern Medical University, Guangzhou  510282, Guangdong Province, China
  • Received:2011-10-01 Revised:2011-12-27 Online:2012-04-08 Published:2012-04-08
  • Contact: author: Li Xiao-rong, Master, Attending physician, Department of Radiology, Guangzhou General Hospital of Guangzhou Military Command of Chinese PLA, Guangzhou 510282, Guangdong Province, China lxrong2008@163.com
  • About author:Ou Shan-xing☆, Doctor, Doctoral supervisor, Professor, Chief physician, Department of Radiology, Guangzhou General Hospital of Guangzhou Military Command of Chinese PLA, Guangzhou 510282, Guangdong Province, China oushanxing@sina.com
  • Supported by:

    Type B Subject Project of the Military Medical Science and Research Plan during “Eleventh Five-Year”, No.2006MB162*; Foundation Program of Traditional Chinese Medicine Department of Guangdong Province, No.20060124*

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Abstract:

BACKGROUND: The prevention of epidural scar formation can reduce the incidence of failed back surgery syndromes. Ozone has been applied in the treatment of lumbar disc herniation, and has achieved good effect.
OBJECTIVE: To investigate the effect of ozone on the healing of wound and hypertrophic scar in rats.
METHODS: Hypertrophic scar models of bilateral circular full-thickness skin wounds were made on the back of rats using surgical incision method, and then the rats were divided into three groups: blank control group with no treatment, oxygen group treated with 5 mL pure oxygen and ozone group treated with 5 mL of 30-40 mg/L ozone. Rats in the latter two groups were injected twice a week; After 4 weeks, hypertrophic scar/granulation tissue samples were extracted. The appearance of hypertrophic scar/granulation tissue as well as the expression of tumor necrosis factor-α and basic fibroblast growth factor in each group were detected by hematoxylin-eosin staining immunohistochemistry.
RESULTS AND CONCLUSION: Compared with the blank control and oxygen groups, the area of hypertrophic scar in the ozone group was smaller after wound healing, besides, there were more epithelia generated and less inflammatory cell infiltration, as well as thinner collagen fibers and more breakages. Furthermore, the expression of tumor necrosis factor α was significantly decreased (P < 0.01), while the expression of basic fibroblast growth factor was significantly increased (P < 0.01). These findings suggest that ozone can reduce inflammatory cell infiltration via its anti-inflammatory effect and decrease the oversynthesis of collagens through inhibiting the expression of tumor necrosis factor α and increasing the expression of basic fibroblast growth factor to inhibit the inflammation of granulation tissue and scar proliferation.
Ou SX, Li XR, Zhang L, Yu ZJ. Effect of ozone on hypertrophic scar during the healing of bilateral circular full-thickness skin wounds on the back of rats. Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(15): 2715-2719.    
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