Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (15): 2681-2685.doi: 10.3969/j.issn.1673-8225.2012.15.005

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Effect of miR-3960 on osteogenic differentiation  

Li Hui1, Pang Ya-ling1, Li Xiao-yan1, Wang Xin-wen2, Wu Gui-fu1, Guo Jian1, Luo Xiang-hang3   

  1. 1Department of Endocrinology, Shaanxi Provincial People’s Hospital, Xi’an  710068, Shaanxi Province, China; 2Department of Orthopedics, Xi’an Red-Cross Hospital, Xi’an  710054, Shaanxi Province, China; 3Institute of Metabolism and Endocrinology, Xiangya Second Hospital of Central South University, Changsha  410011, Hunan Province, China
  • Received:2011-12-31 Revised:2012-02-10 Online:2012-04-08 Published:2012-04-08
  • Contact: author: Luo Xiang-hang, Doctor, Researcher, Doctoral supervisor, Institute of Metabolism and Endocrinology, Xiangya Second Hospital of Central South University, Changsha 410011, Hunan Province, China xianghangluo@126.com
  • About author:Li Hui☆, Doctor, Attending physician, Department of Endocrinology, Shaanxi Provincial People’s Hospital, Xi’an 710068, Shaanxi Province, China sxfylh@163.com
  • Supported by:

    the Natural Science Foundation of Shaanxi Province for the Youth, No. 2011JQ4006*; the National Youth Science Foundation of China, No. 81100208*

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Abstract:

BACKGROUND: Osteoblast differentiation involves highly regulated processes mediated by a large number of hormones and transcription factors. Emerging evidence reveals an additional level of regulation that is mediated by microRNAs. miR-3960 is preferentially expressed in osteoblasts, which suggests that miR-3960 may take part in the regulation process of osteogenic differentiation.
OBJECTIVE: To investigate the role of miR-3960 in osteogenic differentiation of primary mouse bone marrow stromal cells.
METHODS: The parameters of osteoblast differentiation were measured after transfection of bone marrow stromal cells with pSilencer4.1-miR-3960. Osteoblastic differentiation was induced by the addition of 300 μg/L bone morphogenetic protein 2.
RESULTS AND CONCLUSION: Stable and high expression of miR-3960 was obtained in the cells after transfection with pSilencer4.1-miR-3960. Compared with control cells, the levels of alkaline phosphatase activity, osteocalcin secretion and calcium deposition were increased by transfection of pSilencer4.1-miR-3960. Treatment with anti-miR-3960 attenuated the levels of alkaline phosphatase activity and osteocalcin secretion as well as reduced calcium deposition. All these results suggest that miR-3960 promotes osteoblast differentiation of bone marrow stromal cells.

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