Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (15): 2674-2680.doi: 10.3969/j.issn.1673-8225.2012.15.004

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Histological changes of vascularization and osteogenesis in the non-cell based tissue engineered bone promoted by the pedicle fascia flap

Wang Yao-yi 1, Yang Xin-ming2, Hu Zhen-shun1, Meng Xian-yong2, Zhang Ying2, Yin Yan-lin2, Zhang Jun-wei2   

  1. 1Hebei North University, Zhangjiakou  075000, Hebei Province, China; 2Department of Orthopedics, the First Affiliated Hospital of Hebei Northern University, Zhangjiakou  075000, Hebei Province, China
  • Received:2011-10-22 Revised:2011-12-02 Online:2012-04-08 Published:2012-04-08
  • Contact: author: Yang Xin-ming, Chief physician, Professor, Master’s supervisor, Department of Orthopedics, the First Affiliated Hospital of Hebei Northern University, Zhangjiakou 075000, Hebei Province, China yxm1120@sohu.com
  • About author:Wang Yao-yi★, Studying for master’s degree, Hebei North University, Zhangjiakou 075000, Hebei Province, China
  • Supported by:

    Hebei Provincial Key Medical Research Project in 2010 of Hebei Health Department, 20100481*; Hebei Provincial Science and Technology Development Project in 2009 of Science and Technology Department of Hebei Province, Key Medical Research Program, No. 09276102D-46*; Science and Technology Development Mandatory Plan of Zhangjiakou in 2008, No. 0807045D-10*; Research Mandatory Plan of Hebei North University, No. 2009036* 

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Abstract:

BACKGROUND: The pedicle fascia flap promotes the osteogenesis of tissue engineered bone. However, it is rarely reported that weather the promotion effect is achieved through the osteogenic repair process with vascularization-centric in the early stage.
OBJECTIVE: To demonstrate that pedicle fascial flap has a good vascularization effect and can promote osteogenesis by observing the effects of pedicle fascial flap on the vascularization and osteogenesis at different stages of bone defect repair in non-cell based tissue engineered bone.
METHODS: Full-defect rabbit models of ulna long segment bone-periosteum and pedicle fascial flap were constructed and divided into four groups: implantation group, fascia flap without pedicle group, vascular endothelial growth factor group and pedicle fascia flap group. The corresponding materials were implanted into the bone defects..
RESULTS AND CONCLUSION: According to histological observation, the pedicle fascia flap group was superior to the implantation group, fascia flap without pedicle group and vascular endothelial growth factor group not only in the quantity of the new vessels but also in the quantity and quality of the new bone trabeculae at each time point. Vascular regeneration area and new bone trabeculae area of the vascular endothelial growth factor group and pedicle fascia flap group was significantly larger than those of the implantation group and fascia flap without pedicle group in the 4th week after implantation (P < 0.05); the vascular regeneration area and the area of the corresponding new bone trabeculaes in the pedicle fascia flap group was significantly larger than those of the other three groups (P < 0.05). These findings illustrate that pedicle fascia flap has a significant promote effect on the vascularization of the non-cell based tissue engineered bone; the enhanced vascularization is helpful to the osteogenesis, it can effectively improve the quantity and quality of the regenerated bone and shorten the bone repair time.

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