Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (10): 1827-1832.doi: 10.3969/j.issn.1673-8225.2012.10.026

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Herpes simplex virus thymidine kinase expressing mesenchymal stem cells in combination with Ganciclovir in targeted-gene therapy for nasopharyngeal carcinoma  

Zhong Pin-neng, Wen Zhong, Shen Cong-xiang, Wang Hai-li, Wang Jun-qi   

  1. Department of Otolaryngology-Head and Neck Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China
  • Received:2011-11-30 Revised:2012-02-12 Online:2012-03-04 Published:2012-03-04
  • Contact: author: Wen Zhong, M.D., Professor, Department of Otolaryngology-Head and Neck Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China wenzhong60@ 163.com
  • About author:Zhong Pin-neng★, Studying for master’s degree, Department of Otolaryngology- Head and Neck Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China zhongpinneng@163.com
  • Supported by:

    the Science and Technology Development Program of Guangdong Province, No. 2010B031200009*

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Abstract:

BACKGROUND: Recent studies have confirmed that mesenchymal stem cells (MSCs) can be used as targeted-gene delivery vehicles for cancer gene therapy.
OBJECTIVE: To investigate the therapeutic effects of herpes simplex virus thymidine kinase expressing mesenchymal stem cells (TK- MSCs) in combination with Ganciclovir (GCV) on nasopharyngeal carcinoma.
METHODS: The pGL3 -EGFP-TK plasmid was constructed and transfected into Sprague-Dawley rat MSCs by Lipofectamine™ 2000. Cell proliferation was determined by CCK-8 method. Tumor-homing of TK-MSCs was analyzed by Transwell inserts. BALB/C nude mice were inoculated with TK-MSCs to observe the tumorigenicity. Human nasopharyngeal carcinoma cells 5-8F were interfered with TK-MSCs/GCV to investigate the cell viability and the bystander effect.
RESULTS AND CONCLUSION: TK gene was transfected into MSCs successfully. There was no significant difference in cell proliferation between TK-MSCs and MSCs (P > 0.05). TK-MSCs maintained their tumor-homing and had no tumorigenicity. TK-MSCs/GCV significantly inhibited the growth of 5-8F cells (P < 0.01). TK-MSCs/GCV suicide gene therapy system exhibits significant inhibitory effects on growth of 5-8F cells, suggesting that MSCs can be used as delivery vehicles for targete-gene  therapy of nasopharyngeal carcinoma.

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