Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (10): 1833-1836.doi: 10.3969/j.issn.1673-8225.2012.10.027

Previous Articles     Next Articles

Transplantation of bone marrow mesenchymal stem cells improves cognitive function of rats with chronic cerebral ischemia and decreases Nogo-A and NgR protein expression in the hippocampus   

Wang Yan-ling, Yan Yu, Song Bo, Zhang Hui-li, Gong Guang-ming, Chen Si, Fang Hui, Xu Yu-ming   

  1. Third Department of Neurology, First Affiliated Hospital of Zhengzhou University, Zhengzhou   450052, Henan Province, China
  • Received:2012-02-03 Revised:2012-02-13 Online:2012-03-04 Published:2012-03-04
  • Contact: author: Xu Yu-ming, M.D., Professor, Doctoral supervisor, Third Department of Neurology, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China xuyuming@ zzu.edu.cn.
  • About author:Wang Yan-lin★, Studying for master’s degree, Third Department of Neurology, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China 410215135@qq.com
  • Supported by:

     the Major Program of Public Science Research of Henan Province, No. 091100910800*

PDF

410

Abstract:

BACKGROUND: Transplantation of bone marrow mesenchymal stem cells can decrease Nogo-A and NgR expression in rats with spinal cord injury and cerebral infarction.
OBJECTIVE: To investigate the effects of transplantation of bone marrow mesenchymal stem cells on cognitive function of rats with chronic cerebral ischemia and Nogo-A and NgR protein expression in the hippocampus.
METHODS: Thirty Sprague-Dawley rats were randomly divided into sham-surgery, model and bone marrow mesenchymal stem cells groups. Rats in the latter two groups were established into model of chronic cerebral ischemia by permanent ligation of bilateral common carotid artery. In the bone marrow mesenchymal stem cells group, at 7 days after chronic cerebral ischemia induction, bone marrow mesenchymal stem cells (1×107) isolated by ficoll density gradient centrifugation were transfused into rats via the tail vein.
RESULTS AND CONCLUSION: Compared with sham-surgery group, the average escape latency was significantly shortened (P < 0.01), the number that rats passed through the platform was significantly increased (P < 0.05), and Nogo-A and NgR expression in the hippocampal tissue was significantly decreased (P < 0.05) in the bone marrow mesenchymal stem cells group. These findings suggest that transplantation of bone marrow mesenchymal stem cells protects spatial learning and memory abilities of rats with chronic cerebral ischemia. This effect is possibly related to decreased Nogo-A and NgR protein expression.

CLC Number: